| Title: |
Immunoproteasome Inhibition Positively Impacts the Gut-Muscle Axis in Duchenne Muscular Dystrophy |
| Authors: |
Farini A.; Strati F.; Molinaro M.; Mostosi D.; Saccone S.; Tripodi L.; Troisi J.; Landolfi A.; Amoroso C.; Cassani B.; Blanco-Míguez A.; Leonetti E.; Bazzani D.; Bolzan M.; Fortunato F.; Caprioli F.; Facciotti F.; Torrente Y. |
| Contributors: |
Farini, A; Strati, F; Molinaro, M; Mostosi, D; Saccone, S; Tripodi, L; Troisi, J; Landolfi, A; Amoroso, C; Cassani, B; Blanco-Míguez, A; Leonetti, E; Bazzani, D; Bolzan, M; Fortunato, F; Caprioli, F; Facciotti, F; Torrente, Y |
| Publisher Information: |
Springer; DE |
| Publication Year: |
2025 |
| Collection: |
Università degli Studi di Milano-Bicocca: BOA (Bicocca Open Archive) |
| Subject Terms: |
Duchenne muscular dystrophy; immunoproteasome; macrophage; muscle metabolism |
| Description: |
Background: Duchenne Muscular Dystrophy (DMD) features immune-muscle crosstalk, where muscle fibre degeneration enhances pro-inflammatory macrophage infiltration, worsening inflammation and impairing regeneration. Methods: We investigated the impact of immunoproteasome (IP) inhibition on the gut-muscle axis in mdx mice, a well-established model of DMD. We employed microbiota perturbation models, including broad-spectrum antibiotic treatment (ABX) and faecal microbiota transplantation (FMT) from IP-inhibited mdx mice. IP inhibition effects were assessed by analysing gut microbiota composition, intestinal inflammation, muscle integrity and associated metabolic and inflammatory pathways. Results: IP inhibitor ONX-0914 significantly impacted the intestinal inflammatory microenvironment and gut microbiota of mdx mice. ONX-0914 treatment increased gastrointestinal transit (increased wet/dry faecal weights, p = 0.0486 and p = 0.0112, respectively) and partially restored intestinal barrier integrity (reduced FITC-dextran leakage, p = 0.0449). JAM-A was significantly upregulated (p < 0.0001). Colonic CD206+ M2 macrophages increased, while CD68 + M1 cells partially decreased. ONX-0914 downregulated IP isoforms in macrophages (PSMB8: p = 0.0022; PSMB9: p = 0.0186) as well as FOXO-1 (p = 0.0380) and TNF-α (p = 0.0487). Antibiotic-induced microbiota depletion abrogated these effects. Metagenomic analysis revealed significant differences in microbiota composition between C57Bl controls and mdx mice (PERMANOVA p < 0.001), with ONX-0914 inducing enrichment of stachyose degradation pathways. Metabolomic analysis showed enrichment of bacterial metabolites, fatty acid and sugar metabolism pathways, with increased glutathione, galactose, glycerol, glyceraldehyde and TCA cycle intermediates. ONX-0914 improved mitochondrial activity in skeletal muscle, as increased expression of ETC complexes (mdx vs. mdx+ONX: Complex II, p = 0.0338; Complex IV, p = 0.0023) and TCA enzymes (mdx vs. FTMmdx+ONX: IDH p = 0.0258; FH p = 0.0366). ... |
| Document Type: |
article in journal/newspaper |
| File Description: |
STAMPA |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/41035224; info:eu-repo/semantics/altIdentifier/wos/WOS:001602613400023; volume:16; issue:5; journal:JOURNAL OF CACHEXIA, SARCOPENIA AND MUSCLE; https://hdl.handle.net/10281/576208 |
| DOI: |
10.1002/jcsm.70054 |
| Availability: |
https://hdl.handle.net/10281/576208; https://doi.org/10.1002/jcsm.70054 |
| Rights: |
info:eu-repo/semantics/openAccess ; license:Creative Commons ; license uri:http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.8203DCDC |
| Database: |
BASE |