| Title: |
The osteoconductive properties of graphene-based material surfaces are finely tuned by the conditioning layer and surface chemistry |
| Authors: |
Tiberio F.; Desiderio C.; Perini G.; Moretti I.; Cui L.; Salvati M.; Polito L.; Parolini O.; De Spirito M.; Lattanzi W.; Papi M.; Di Pietro L.; Palmieri V. |
| Contributors: |
Tiberio, Federica; Amato, F.; Desiderio, Claudia; Vincenzoni, F.; Perini, Giordano; Moretti, Irene; Augello, A.; Friggeri, G.; Cui, Lishan; Giaccari, L.; Salvati, Martina; Polito, Luca; Parolini, Ornella; De Spirito, Marco; Marrani, A. G.; Lattanzi, Wanda; Papi, Massimiliano; Di Pietro, Lorena; Palmieri, Valentina |
| Publisher Information: |
Royal Society of Chemistry |
| Publication Year: |
2024 |
| Collection: |
Università Cattolica del Sacro Cuore: PubliCatt |
| Subject Terms: |
graphene; molecular layer; osteogenesis; cells; stem cells; Settore BIO/13 - BIOLOGIA APPLICATA |
| Description: |
The molecular layer that adsorbs on the biomaterial surface upon contacting body tissues and fluids, termed the conditioning layer (CL), influences cell behavior regulating scaffold integration and resilience in a patient-specific fashion. To predict and improve the clinical outcome of 3D-printed scaffolds, graphene coatings are employed in bone tissue engineering, due to the possibility to functionalize its chemical/physical properties. In this study, we investigated the composition and the influence of the CL on three different graphene oxide-based coatings of 3D-printed polycaprolactone (PCL) implants: graphene oxide (-GO), carboxylated GO (-GO-COOH) and reduced GO (-rGO). The effects of surface features and CL were evaluated in vitro using bone marrow-derived mesenchymal stromal cells (hBM-MSC). Our results showed that the CL formed on negatively charged PCL-GO-COOH and PCL-rGO scaffolds reduced cell adhesion, while simultaneously enhancing cell cluster formation and proliferation by a fivefold increase. The quantification of bone mineralized matrix highlighted that CL on both PCL-GO-COOH and PCL-rGO coatings sustained the osteogenic potential of these two types of GO. The analysis of CL components adsorbed on the scaffolds revealed that the PCL-GO-COOH and PCL-rGO coatings tend to entrap specific patterns of serum proteins (e.g. anti-adhesive and osteogenic modulators) and ions (carbonate and phosphate), suggesting a correlation between these enriched components and the observed biological outcomes of conditioned scaffolds. Lastly, PCL-rGO coatings maintained unique antibacterial properties after in vitro simulated CL formation, representing a suitable promising strategy to improve bone grafting capable of shaping CL formation while preserving the favorable osteoinductive properties of scaffolds. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/wos/WOS:001208471600001; volume:5; issue:11; firstpage:4772; lastpage:4785; numberofpages:14; issueyear:2024; journal:MATERIALS ADVANCES; https://hdl.handle.net/10807/287218 |
| DOI: |
10.1039/d4ma00144c |
| Availability: |
https://hdl.handle.net/10807/287218; https://doi.org/10.1039/d4ma00144c |
| Rights: |
info:eu-repo/semantics/openAccess ; license:Creative commons ; license uri:http://creativecommons.org/licenses/by-nc/4.0/ |
| Accession Number: |
edsbas.8279750F |
| Database: |
BASE |