| Title: |
The ultra high sensitivity blood counter: a compact, MRI-compatible, radioactivity counter for pharmacokinetic studies in μl volumes |
| Authors: |
Convert, L.; Sarrhini, Otman; Paillé, Maxime; Salem, Nicolas; Charette, Paul; Lecomte, Roger |
| Contributors: |
Sherbrooke Molecular Imaging Center of CRCHUS and Department of Nuclear Medecine and Radiobiology (CIMS); Université de Sherbrooke = University of Sherbrooke Sherbrooke (UdeS); Laboratoire Nanotechnologies et Nanosystèmes Sherbrooke (LN2); Université de Sherbrooke = University of Sherbrooke Sherbrooke (UdeS)-École Centrale de Lyon (ECL); Université de Lyon-Université de Lyon-École Supérieure de Chimie Physique Électronique de Lyon (CPE)-Institut National des Sciences Appliquées de Lyon (INSA Lyon); Université de Lyon-Institut National des Sciences Appliquées (INSA)-Institut National des Sciences Appliquées (INSA)-Centre National de la Recherche Scientifique (CNRS)-Université Grenoble Alpes (UGA); Institut Interdisciplinaire d'Innovation Technologique Sherbrooke (3IT); Biogen Cambridge, Massachusetts, USA |
| Source: |
EISSN: 2057-1976 ; Biomedical Physics & Engineering Express ; https://hal.science/hal-03701266 ; Biomedical Physics & Engineering Express, 2022, 8 (3), pp.035022. ⟨10.1088/2057-1976/ac4c29⟩ |
| Publisher Information: |
CCSD; IOP Publishing |
| Publication Year: |
2022 |
| Collection: |
Université de Lyon: HAL |
| Subject Terms: |
[SPI]Engineering Sciences [physics] |
| Description: |
International audience ; Abstract Quantification of physiological parameters in preclinical pharmacokinetic studies based on nuclear imaging requires the monitoring of arterial radioactivity over time, known as the arterial input function (AIF). Continuous derivation of the AIF in rodent models is very challenging because of the limited blood volume available for sampling. To address this challenge, an Ultra High Sensitivity Blood Counter (UHS-BC) was developed. The device detects beta particles in real-time using silicon photodiodes, custom low-noise electronics, and 3D-printed plastic cartridges to hold standard catheters. Two prototypes were built and characterized in two facilities. Sensitivities up to 39% for 18 F and 58% for 11 C-based positron emission tomography (PET) tracers were demonstrated. 99m Tc and 125 I based Single Photon Emission Computed Tomography (SPECT) tracers were detected with greater than 3% and 10% sensitivity, respectively, opening new applications in nuclear imaging and fundamental biology research. Measured energy spectra show all relevant peaks down to a minimum detectable energy of 20 keV. The UHS-BC was shown to be highly reliable, robust towards parasitic background radiation and electromagnetic interference in the PET or MRI environment. The UHS-BC provides reproducible results under various experimental conditions and was demonstrated to be stable over days of continuous operation. Animal experiments showed that the UHS-BC performs accurate AIF measurements using low detection volumes suitable for small animal models in PET, SPECT and PET/MRI investigations. This tool will help to reduce the time and number of animals required for pharmacokinetic studies, thus increasing the throughput of new drug development. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1088/2057-1976/ac4c29 |
| Availability: |
https://hal.science/hal-03701266; https://hal.science/hal-03701266v1/document; https://hal.science/hal-03701266v1/file/article_10.pdf; https://doi.org/10.1088/2057-1976/ac4c29 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.830656D3 |
| Database: |
BASE |