| Title: |
ARF1.GTP, tyrosine-based signals, and phosphatidylinositol 4,5-bisphosphate constitute a minimal machinery to recruit the AP-1 clathrin adaptor to membranes |
| Authors: |
Crottet, Pascal; Meyer, Daniel M.; Rohrer, Jack; Spiess, Martin |
| Source: |
ISSN:1939-4586 ; ISSN:1059-1524 ; Molecular Biology of the Cell. |
| Publisher Information: |
American Society for Cell Biology |
| Publication Year: |
2002 |
| Collection: |
ZHAW digitalcollection (Repository of the Zurich University of Applied Sciences) |
| Subject Terms: |
info:eu-repo/classification/ddc/571; info:eu-repo/classification/ddc/572 |
| Description: |
At the trans-Golgi network, clathrin coats containing AP-1 adaptor complexes are formed in an ARF1-dependent manner, generating vesicles transporting cargo proteins to endosomes. The mechanism of site-specific targeting of AP-1 and the role of cargo are poorly understood. We have developed an in vitro assay to study the recruitment of purified AP-1 adaptors to chemically defined liposomes presenting peptides corresponding to tyrosine-based sorting motifs. AP-1 recruitment was found to be dependent on myristoylated ARF1, GTP or nonhydrolyzable GTP-analogs, tyrosine signals, and small amounts of phosphoinositides, most prominently phosphatidylinositol 4,5-bisphosphate, in the absence of any additional cytosolic or membrane bound proteins. AP-1 from cytosol could be recruited to a tyrosine signal independently of the lipid composition, but the rate of recruitment was increased by phosphatidylinositol 4,5-bisphosphate. The results thus indicate that cargo proteins are involved in coat recruitment and that the local lipid composition contributes to specifying the site of vesicle formation. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://hdl.handle.net/11475/6813; https://digitalcollection.zhaw.ch/handle/11475/6813 |
| DOI: |
10.1091/mbc.e02-05-0309 |
| Availability: |
https://hdl.handle.net/11475/6813; https://doi.org/10.1091/mbc.e02-05-0309; https://digitalcollection.zhaw.ch/handle/11475/6813 |
| Rights: |
Licence according to publishing contract |
| Accession Number: |
edsbas.8512F1F3 |
| Database: |
BASE |