| Description: |
The aim of this study is to determine the isolation rate of S. aureus in nasal cavities of healthy cattle, antibiotic resistance profiles of the isolates and to investigate genes associated with antibiotic resistance. For this purpose; 250 nasal swab samples collected from healthy dairy cattle in farms located in the provinces of Istanbul, Bursa, K & imath;rklareli and Tekirda & gbreve;), examined for the presence S. aureus . S.aureus was isolated from 7.6% (n=19) of the examined cows. Antibiotic susceptibility of isolates was determined by disc diffusion method. The isolates were resistant to penicillin (78.95%); tetracycline (68.42%); erythromycin (63.16%); doxycycline (36.84%); cefaperazone (31.58%); cefoxitin (26.32%); ceftiofur (26.32%) and clindamycin (21.05%), while all were sensitive to penicillin-novobiocin, linezolid, quinupristin-dalfopristin, chloramphenicol, gentamicin, trimethoprim-sulfamethoxazole and enrofloxacin. Five of the 12 isolates resistant to erythromycin showed inducible clindamycin resistance. Antibiotic resistance genes and pvl gene were examined by Polymerase Chain Reaction method. The most common genes detected in the isolates were blaZ (93,33%), mecA (52.63%), tetL (23,08%), tetK (53,85%), ermB (8,33%) and ermC (41,67%). The ermA , tetM , tetO and pvl genes were not detected in any of the isolates. In our study, the most common type of SCC mec was found to be type IV 90% (n=9) and only one isolate type I 10% (n=1). ; Acknowledgements This study is a part of Master Thesis study titled Characterization of Staphylococcus aureus Strains Isolated from Nasal Mucosa of Cows by Veterinari- an Hager ABUGHONEM |