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Atypical Mitochondrial Phenotype of Colonic CD4+ and CD8+ T Cells During Experimental Chronic Colitis

Title: Atypical Mitochondrial Phenotype of Colonic CD4+ and CD8+ T Cells During Experimental Chronic Colitis
Authors: Negele, Jonas; Franz, Tobias; Krone, Anna; Roder, Marc; Gelmez, Elif; Jantz-Naeem, Nouria; Kershaw, Olivia; Keitel-Anselmino, Verena; Jeron, Andreas; Bruder, Dunja; Kahlfuß, Sascha
Publication Year: 2025
Collection: FU Berlin: Refubium
Subject Terms: ulcerative colitis; inflammatory bowel disease; t cells; b cells; neutrophils; intestinal epithelial cells; DSS colitis; autoimmune colitis; ddc:616
Description: Background: Patients with ulcerative colitis (UC) suffer from a chronic relapsing-remitting inflammatory bowel disease and show heterogeneous disease severity and therapy response. It was recently reported that rectal biopsies from patients with UC show a downregulation of various mitochondrial genes during active UC. Methods: We used dextran sulfate sodium (DSS)-induced colitis as a preclinical mouse model for UC to study metabolic characteristics of ex vivo colonic lamina propria CD4+ and CD8+ T cells, B cells, eosinophils, neutrophils and intestinal epithelial cells during experimental acute and chronic inflammatory bowel disease and remission state. Results: Our results indicate that CD4+ and CD8+ T cells in the colon produce significantly less mitochondrial reactive oxygen species and possess smaller mitochondria during chronic DSS-induced colitis, which is resolved during remission state. In addition, CD4+ and CD8+ T cells exhibit increased glucose uptake during acute but defective glucose consumption during chronic DSS colitis. Conclusions: Together, our data provide evidence for an atypical mitochondrial phenotype of colonic CD4+ and CD8+ T cells during chronic colitis that is resolved during the remission phase of the disease.
Document Type: article in journal/newspaper
File Description: 17 Seiten; application/pdf
Language: English
DOI: 10.17169/refubium-50247
DOI: 10.3390/biomedicines13092094
Availability: https://refubium.fu-berlin.de/handle/fub188/50520; https://doi.org/10.17169/refubium-50247; https://doi.org/10.3390/biomedicines13092094
Rights: https://creativecommons.org/licenses/by/4.0/
Accession Number: edsbas.853C86FD
Database: BASE