| Title: |
Epidemiologic, Immunologic, and Virus Characteristics in Patients With Paired Severe Acute Respiratory Syndrome Coronavirus 2 Serology and Reverse-Transcription Polymerase Chain Reaction Testing |
| Authors: |
Shragai, Talya; Smith-Jeffcoat, Sarah E; Koh, Mitsuki; Schechter, Marcos C; Rebolledo, Paulina A; Kasinathan, Vyjayanti; Wang, Yun; Hoffman, Adam; Miller, Halie; Tejada-Strop, Alexandra; Jain, Shilpi; Tamin, Azaibi; Harcourt, Jennifer L; Thornburg, Natalie J; Wong, Phili; Medrzycki, Magdalena; Folster, Jennifer M; Semenova, Vera; Steward-Clark, Evelene; Drobenuic, Jan; Biedron, Caitlin; Stewart, Rebekah J; da Silva, Juliana; Kirking, Hannah L; Tate, Jacqueline E; Adeyemo, AdeSubomi O; Moorman, Anne; Bauman, Brenda L; Joseph, Kahaliah; O’Hegarty, Michelle; Kamal, Nazia; Cohen, Mila; Rosetti, Rebecca; Britton, Amadea; Aholou, Tiffiany M; Callahan, Courtney T; Fonseka, Jamila; Agyemang, Elfriede; Lawson, Miriam J; Deutsch-Feldman, Molly; Tiwari, Tejpratap S P; Sami, Samira; Tao, Hong |
| Contributors: |
CDC; CDC Foundation |
| Source: |
The Journal of Infectious Diseases ; volume 225, issue 2, page 229-237 ; ISSN 0022-1899 1537-6613 |
| Publisher Information: |
Oxford University Press (OUP) |
| Publication Year: |
2021 |
| Description: |
Background The natural history and clinical progression of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infections can be better understood using combined serological and reverse-transcription polymerase chain reaction (RT-PCR) testing. Methods Nasopharyngeal swabs and serum were collected at a single time-point from patients at an urban, public hospital during August–November 2020 and tested for SARS-CoV-2 using RT-PCR, viral culture, and anti-spike pan-immunoglobulin antibody testing. Participant demographics and symptoms were collected through interview. The χ 2 and Fisher exact tests were used to identify associations between RT-PCR and serology results with presence of viable virus and frequency of symptoms. Results Among 592 participants, 129 (21.8%) had evidence of SARS-CoV-2 infection by RT-PCR or serology. Presence of SARS-CoV-2 antibodies was strongly associated with lack of viable virus (P = .016). COVID-19 symptom frequency was similar for patients testing RT-PCR positive/seronegative and patients testing RT-PCR positive/seropositive. Patients testing RT-PCR positive/seronegative reported headaches, fatigue, diarrhea, and vomiting at rates not statistically significantly different from those testing RT-PCR negative/seropositive. Conclusions While patients testing SARS-CoV-2 seropositive were unlikely to test positive for viable virus and were therefore at low risk for forward transmission, coronavirus disease 2019 (COVID-19) symptoms were common. Paired SARS-CoV-2 RT-PCR and antibody testing provides more nuanced understanding of patients’ COVID-19 status. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1093/infdis/jiab349 |
| DOI: |
10.1093/infdis/jiab349/39619914/jiab349.pdf |
| Availability: |
https://doi.org/10.1093/infdis/jiab349; http://academic.oup.com/jid/advance-article-pdf/doi/10.1093/infdis/jiab349/39619914/jiab349.pdf; https://academic.oup.com/jid/article-pdf/225/2/229/42224086/jiab349.pdf |
| Rights: |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
| Accession Number: |
edsbas.863558CF |
| Database: |
BASE |