| Title: |
NASCarD (Nanopore Adaptive Sampling with Carrier DNA): A Rapid, PCR-Free Method for SARS-CoV-2 Whole-Genome Sequencing in Clinical Samples. |
| Authors: |
Terrazos Miani, Miguel A; Borcard, Loïc; Gempeler, Sonja; Baumann, Christian; Bittel, Pascal; Leib, Stephen L; Neuenschwander, Stefan; Ramette, Alban Nicolas |
| Source: |
Terrazos Miani, Miguel A; Borcard, Loïc; Gempeler, Sonja; Baumann, Christian; Bittel, Pascal; Leib, Stephen L; Neuenschwander, Stefan; Ramette, Alban Nicolas (2024). NASCarD (Nanopore Adaptive Sampling with Carrier DNA): A Rapid, PCR-Free Method for SARS-CoV-2 Whole-Genome Sequencing in Clinical Samples. Pathogens, 13(1) MDPI AG 10.3390/pathogens13010061 |
| Publisher Information: |
MDPI AG |
| Publication Year: |
2024 |
| Collection: |
BORIS (Bern Open Repository and Information System, University of Bern) |
| Subject Terms: |
570 Life sciences; biology; 610 Medicine & health |
| Description: |
Whole-genome sequencing (WGS) represents the main technology for SARS-CoV-2 lineage characterization in diagnostic laboratories worldwide. The rapid, near-full-length sequencing of the viral genome is commonly enabled by high-throughput sequencing of PCR amplicons derived from cDNA molecules. Here, we present a new approach called NASCarD (Nanopore Adaptive Sampling with Carrier DNA), which allows a low amount of nucleic acids to be sequenced while selectively enriching for sequences of interest, hence limiting the production of non-target sequences. Using COVID-19 positive samples available during the omicron wave, we demonstrate how the method may lead to >99% genome completeness of the SARS-CoV-2 genome sequences within 7 h of sequencing at a competitive cost. The new approach may have applications beyond SARS-CoV-2 sequencing for other DNA or RNA pathogens in clinical samples. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
https://boris.unibe.ch/191977/ |
| Availability: |
https://boris.unibe.ch/191977/1/pathogens-13-00061.pdf; https://boris.unibe.ch/191977/ |
| Rights: |
info:eu-repo/semantics/openAccess |
| Accession Number: |
edsbas.868EAF43 |
| Database: |
BASE |