| Title: |
Chicken cGAS senses fowlpox virus infection and regulates macrophage effector functions |
| Authors: |
Oliveira, M; Rodrigues, DR; Guillory, V; Kut, E; Giotis, ES; Skinner, MA; Guabiraba, R; Bryant, CE; Ferguson, BJ |
| Contributors: |
Biotechnology and Biological Sciences Research Council (BBSRC) |
| Publisher Information: |
Frontiers Media |
| Publication Year: |
2020 |
| Collection: |
Imperial College London: Spiral |
| Subject Terms: |
1107 Immunology; 1108 Medical Microbiology |
| Description: |
The anti-viral immune response is dependent on the ability of infected cells to sense foreign nucleic acids. In multiple species, the pattern recognition receptor (PRR) cyclic GMP-AMP synthase (cGAS) senses viral DNA as an essential component of the innate response. cGAS initiates a range of signaling outputs that are dependent on generation of the second messenger cGAMP that binds to the adaptor protein stimulator of interferon genes (STING). Here we show that in chicken macrophages, the cGAS/STING pathway is essential not only for the production of type-I interferons in response to intracellular DNA stimulation, but also for regulation of macrophage effector functions including the expression of MHC-II and co-stimulatory molecules. In the context of fowlpox, an avian DNA virus infection, the cGAS/STING pathway was found to be responsible for type-I interferon production and MHC-II transcription. The sensing of fowlpox virus DNA is therefore essential for mounting an anti-viral response in chicken cells and for regulation of a specific set of macrophage effector functions. |
| Document Type: |
article in journal/newspaper |
| Language: |
unknown |
| Relation: |
Frontiers in Immunology; http://hdl.handle.net/10044/1/86317; BB/E009956/1; BB/G018545/1; BB/H005323/1; BB/K002465/1 |
| DOI: |
10.3389/fimmu.2020.613079 |
| Availability: |
http://hdl.handle.net/10044/1/86317; https://doi.org/10.3389/fimmu.2020.613079 |
| Rights: |
© 2021 Oliveira, Rodrigues, Guillory, Kut, Giotis, Skinner, Guabiraba, Bryant and Ferguson. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. ; https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.89B248D0 |
| Database: |
BASE |