| Title: |
Macrophage-specific expression of group IIA sPLA(2) results in accelerated atherogenesis by increasing oxidative stress |
| Authors: |
Tietge, UJF; Pratico, D; Ding, T; Funk, CD; Hildebrand, RB; Van Berkel, TJ; Van Eck, M |
| Source: |
Tietge, UJF, Pratico, D, Ding, T, Funk, CD, Hildebrand, RB, Van Berkel, TJ & Van Eck, M 2005, 'Macrophage-specific expression of group IIA sPLA(2) results in accelerated atherogenesis by increasing oxidative stress', Journal of Lipid Research, vol. 46, no. 8, pp. 1604-1614. https://doi.org/10.1194/jlr.M400469-JLR200 |
| Publication Year: |
2005 |
| Collection: |
University of Groningen research database |
| Subject Terms: |
secretory phospholipase A(2); lipoxygenase; hypercholesterolemia; inflammation; lipoproteins; LOW-DENSITY-LIPOPROTEIN; HUMAN AORTIC PROTEOGLYCANS; RECEPTOR-DEFICIENT MICE; TRANSGENIC MICE; PLASMA-LEVELS; GENE FAMILY; IN-VIVO; ATHEROSCLEROSIS; DISEASE |
| Description: |
Group IIA secretory phospholipase A(2) (sPLA(2)) isan acute-phase protein mediating decreased plasma HDL cholesterol and increased atherosclerosis. This study investigated the impact of macrophage-specific sPLA(2) overexpression on lipoprotein metabolism and atherogenesis. Macrophages from sPLA(2) transgenic mice have 2.5 times increased rates of LDL oxidation ( thiobarbituric acid-reactive substances formation) in vitro ( 59 +/- 5 vs. 24 +/- 4 nmol malon-dialdehyde/ mg protein; P LDLR-/-; n = 19) or wild-type C57BL/6 littermates (C57 BL/6 -> LDLR-/-; n = 19) and maintained for 8 weeks on chow and then for 9 weeks on a Western-type diet. Plasma sPLA 2 activity and plasma lipoprotein profiles were not significantly different between sPLA(2). LDLR-/- and C57BL/ 6 -> LDLR-/- mice. Aortic root atherosclerosis was increased by 57% in sPLA(2). LDLR-/- mice compared with C57BL/ 6 -> LDLR-/- controls ( P |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| ISSN: |
0022-2275 |
| Relation: |
info:eu-repo/semantics/altIdentifier/wos/000230597400005; info:eu-repo/semantics/altIdentifier/hdl/https://hdl.handle.net/11370/318dc6c1-5dbb-452a-a5b4-650683f92320; info:eu-repo/semantics/altIdentifier/pissn/0022-2275 |
| DOI: |
10.1194/jlr.M400469-JLR200 |
| Availability: |
https://hdl.handle.net/11370/318dc6c1-5dbb-452a-a5b4-650683f92320; https://research.rug.nl/en/publications/318dc6c1-5dbb-452a-a5b4-650683f92320; https://doi.org/10.1194/jlr.M400469-JLR200 |
| Rights: |
info:eu-repo/semantics/restrictedAccess |
| Accession Number: |
edsbas.89DF1939 |
| Database: |
BASE |