| Title: |
Biofilm-Formation Ability and the Presence of Adhesion Genes in Coagulase-Negative Staphylococci Isolates from Chicken Broilers |
| Authors: |
Agnieszka Marek; Ewelina Pyzik; Dagmara Stępień-Pyśniak; Marta Dec; Łukasz S. Jarosz; Anna Nowaczek; Magdalena Sulikowska |
| Source: |
Animals, Vol 11, Iss 3, p 728 (2021) |
| Publisher Information: |
MDPI AG |
| Publication Year: |
2021 |
| Collection: |
Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: |
biofilm; biofilm-associated genes; coagulase-negative staphylococci; broiler chickens; Veterinary medicine; SF600-1100; Zoology; QL1-991 |
| Description: |
The aim of the study was to analyze the biofilm-production capacity of 87 coagulase-negative Staphylococcus strains (CoNS) isolated from broiler chickens and to determine the occurrence of biofilm-associated genes. The biofilm production capacity of staphylococci was assessed using the microtiter plate method (MTP), and the frequency of genes was determined by PCR. The ability to form a biofilm in vitro was shown in 79.3% of examined strains. Strong biofilm capacity was demonstrated in 26.4% of strains, moderate capacity in 25.3%, weak capacity in 27.6%, and a complete lack of biofilm production capacity in 20.7% of strains. The ica AB gene responsible for the production of extracellular polysaccharide adhesins was detected in 6.9% of strains. The other four genes, i.e., bap (encoding biofilm-associated protein), atl E (encoding cell surface protein exhibiting vitronectin-binding activity), fbe (encoding fibrinogen-binding protein), and eno (encoding laminin-binding protein) were detected in 5.7%, 19.5%, 8%, and 70.1% of strains, respectively. Demonstration of genes that play a role in bacterial biofilm formation may serve as a genetic basis to distinguish between symbiotic and potentially invasive coagulase-negative staphylococcal strains. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://www.mdpi.com/2076-2615/11/3/728; https://doaj.org/toc/2076-2615; https://doaj.org/article/968bdaa78d6f435f852cc79c51379dd6 |
| DOI: |
10.3390/ani11030728 |
| Availability: |
https://doi.org/10.3390/ani11030728; https://doaj.org/article/968bdaa78d6f435f852cc79c51379dd6 |
| Accession Number: |
edsbas.8B009EDD |
| Database: |
BASE |