| Title: |
Exploiting Connections for Viral Replication |
| Authors: |
Wong, LH; Edgar, JR; Martello, A; Ferguson, BJ; Eden, ER |
| Source: |
Frontiers in Cell and Developmental Biology , 9 , Article 640456. (2021) |
| Publisher Information: |
FRONTIERS MEDIA SA |
| Publication Year: |
2021 |
| Collection: |
University College London: UCL Discovery |
| Subject Terms: |
Membrane contact sites (MCS); double membrane vesicles (DMVs); SARS-CoV-2; viral replication; lipid transport |
| Description: |
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the cause of the COVID-19 (coronavirus disease 2019) pandemic, is a positive strand RNA (+RNA) virus. Like other +RNA viruses, SARS-CoV-2 is dependent on host cell metabolic machinery to survive and replicate, remodeling cellular membranes to generate sites of viral replication. Viral RNA-containing double-membrane vesicles (DMVs) are a striking feature of +RNA viral replication and are abundant in SARS-CoV-2–infected cells. Their generation involves rewiring of host lipid metabolism, including lipid biosynthetic pathways. Viruses can also redirect lipids from host cell organelles; lipid exchange at membrane contact sites, where the membranes of adjacent organelles are in close apposition, has been implicated in the replication of several +RNA viruses. Here we review current understanding of DMV biogenesis. With a focus on the exploitation of contact site machinery by +RNA viruses to generate replication organelles, we discuss evidence that similar mechanisms support SARS-CoV-2 replication, protecting its RNA from the host cell immune response. |
| Document Type: |
article in journal/newspaper |
| File Description: |
text |
| Language: |
English |
| Relation: |
https://discovery.ucl.ac.uk/id/eprint/10128072/ |
| Availability: |
https://discovery.ucl.ac.uk/id/eprint/10128072/1/fcell-09-640456.pdf; https://discovery.ucl.ac.uk/id/eprint/10128072/ |
| Rights: |
open |
| Accession Number: |
edsbas.8BBAE86A |
| Database: |
BASE |