| Title: |
Telomere length and age-dependent telomere attrition: the blood-and-muscle model |
| Authors: |
Chahine, Mirna; Toupance, Simon; El-Hakim, Sandy; Labat, Carlos; Gautier, Sylvie; Moussallem, Toufic; Yared, Pierre; Asmar, Roland; Benetos, Athanase |
| Contributors: |
Neuroscience Research Center Beirut, Lebanon (Faculty of Medical Sciences); الجامعة اللبنانية بيروت = Lebanese University Beirut = Université libanaise Beyrouth (LU / ULB); Défaillance Cardiovasculaire Aiguë et Chronique (DCAC); Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Lorraine (UL); Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy); Nancyclotep- Experimental Imaging Platform = Plate-forme d'imagerie moléculaire; Centre Hospitalier Régional Universitaire de Nancy (CHRU Nancy)-Université de Lorraine (UL); Faculty of Public Health Fanar, Liban; Partially supported by a grant to Athanase Benetos from the French National Research Agency; Foundation-Medical Research Institute (F-MRI®) for partial financial support of this study |
| Source: |
ISSN: 0008-4212. |
| Publisher Information: |
CCSD; NRC Research Press |
| Publication Year: |
2019 |
| Collection: |
Université de Lorraine: HAL |
| Subject Terms: |
Southern blot; aging; buvardage de Southern; leucocytes; leukocytes; muscle; télomère; vieillissement; [SDV]Life Sciences [q-bio]; [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology; [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system; [SDV.BBM]Life Sciences [q-bio]/Biochemistry; Molecular Biology |
| Description: |
International audience ; Short telomere length (TL) is associated with atherosclerotic cardiovascular disease (ACVD) and other age-related diseases. It is unclear whether these associations originate from having inherently short TL or a faster TL attrition before or during disease development. We proposed the blood-and-muscle model to assess TL dynamics throughout life course. Our objective was to measure TL in leukocytes (LTL) and in skeletal muscle (MTL), which served as a proxy of TL at birth. The delta (MTL-LTL) represented life-long telomere attrition. Blood draws and skeletal muscle biopsies were performed on 35 Lebanese individuals undergoing surgery. Following DNA extraction, LTL and MTL were measured by Southern blot. In every individual aged between 30 and 85 years, MTL was longer than LTL. With age, MTL and LTL decreased, but the delta (MTL-LTL) increased by 14 bp/year. We validated the blood-and-muscle model that allowed us to identify TL, TL at birth, and lifelong TL attrition in a cross-sectional study. This model can be used in larger cross-sectional studies to evaluate the association of telomere dynamics with age-related diseases onset and progression. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/30874445; PUBMED: 30874445 |
| DOI: |
10.1139/cjpp-2018-0582 |
| Availability: |
https://hal.univ-lorraine.fr/hal-02519164; https://hal.univ-lorraine.fr/hal-02519164v1/document; https://hal.univ-lorraine.fr/hal-02519164v1/file/cjpp-2018-0582.pdf; https://doi.org/10.1139/cjpp-2018-0582 |
| Rights: |
info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.8BF32FE9 |
| Database: |
BASE |