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Phylogenomic Barcoding of Soil Seed Bank–Persistent and Wind-Dispersed Non-Native Plant Species in South Georgia

Title: Phylogenomic Barcoding of Soil Seed Bank–Persistent and Wind-Dispersed Non-Native Plant Species in South Georgia
Authors: Viruel, J; Sweeney, CJ; Day, R; White, K; Dawson, W; Myer, B; Floyd, K; Corcoran, M; Kelting, C; Poncet, S; Forest, F; Clubbe, C; Newton, RJ
Publisher Information: Wiley
Publication Year: 2026
Collection: The University of Liverpool Repository
Description: Climate change and invasive species are leading drivers of biodiversity loss, with island ecosystems being especially vulnerable. South Georgia, a remote sub-Antarctic island, is 170 km long with approximately 30,000 ha of vegetated coastal areas, as snow and ice dominate the inland regions. Human activities on the island have historically introduced non-native species, resulting in 41 introduced vascular plant species compared with only 24 native ones. To address this imbalance, the South Georgia Non-Native Plant Management Strategy was implemented (2016–2020) to control non-native plant populations. We assessed emergent seedlings from South Georgia soil samples and wind-dispersed seeds to determine which species persist in the soil seed bank and contribute to dispersal. Using a molecular barcoding approach, we evaluated traditional markers (rbcL and matK) and optimized a high-throughput Angiosperms353 sequencing pipeline for accurate seedling identification. We generated a reference library covering all native and non-native species and applied this to 1,498 emergent seedlings and 737 trapped seeds. Molecular barcoding identified 21 species, including 10 non-natives and 11 natives. Strikingly, 84% of emergent seedlings were non-native, with Class III invasive species (Cerastium fontanum, Poa annua, Taraxacum officinale) dominating across most sites and in all wind traps. By contrast, Class I and II species occurred rarely and only at a few sites, indicating that management efforts have substantially reduced their spread, though viable seeds persist in the soil. These findings highlight both the continued threat from persistent seed banks of dominant invaders and the value of molecular barcoding for long-term monitoring. Our approach provides a framework for biosecurity and restoration management in South Georgia and other vulnerable ecosystems under climate change pressures.
Document Type: article in journal/newspaper
Language: English
ISSN: 1755-098X
Relation: Collapse authors list. Viruel, J orcid:0000-0001-5658-8411 , Sweeney, CJ, Day, R, White, K, Dawson, W orcid:0000-0003-3402-0774 , Myer, B, Floyd, K, Corcoran, M, Kelting, C, Poncet, S et al (show 3 more authors) , Forest, F, Clubbe, C and Newton, RJ (2026) Phylogenomic Barcoding of Soil Seed Bank–Persistent and Wind-Dispersed Non-Native Plant Species in South Georgia Molecular Ecology Resources, 26 (1). e70068-. ISSN 1755-098X, 1755-0998
DOI: 10.1111/1755-0998.70068
Availability: https://livrepository.liverpool.ac.uk/3195565/; https://doi.org/10.1111/1755-0998.70068
Accession Number: edsbas.8CD1607F
Database: BASE