| Title: |
Diagnostics of BAP1-Tumor Predisposition Syndrome by a Multitesting Approach: A Ten-Year-Long Experience |
| Authors: |
Sculco, Marika; La Vecchia, Marta; Aspesi, Anna; Clavenna, Michela Giulia; Salvo, Michela; Borgonovi, Giulia; Pittaro, Alessandra; Witel, Gianluca; Napoli, Francesca; Listì, Angela; Grosso, Federica; Libener, Roberta; Maconi, Antonio; Rena, Ottavio; Boldorini, Renzo; Giachino, Daniela; Bironzo, Paolo; Maffè, Antonella; Alì, Greta; Elefanti, Lisa; Menin, Chiara; Righi, Luisella; Tampieri, Cristian; Scagliotti, Giorgio Vittorio; Dianzani, Caterina; Ferrante, Daniela; Migliore, Enrica; Magnani, Corrado; Mirabelli, Dario; Matullo, Giuseppe; Dianzani, Irma |
| Contributors: |
Sculco, Marika; La Vecchia, Marta; Aspesi, Anna; Clavenna, Michela Giulia; Salvo, Michela; Borgonovi, Giulia; Pittaro, Alessandra; Witel, Gianluca; Napoli, Francesca; Listì, Angela; Grosso, Federica; Libener, Roberta; Maconi, Antonio; Rena, Ottavio; Boldorini, Renzo; Giachino, Daniela; Bironzo, Paolo; Maffè, Antonella; Alì, Greta; Elefanti, Lisa; Menin, Chiara; Righi, Luisella; Tampieri, Cristian; Scagliotti, Giorgio Vittorio; Dianzani, Caterina; Ferrante, Daniela; Migliore, Enrica; Magnani, Corrado; Mirabelli, Dario; Matullo, Giuseppe; Dianzani, Irma |
| Publication Year: |
2022 |
| Collection: |
Università degli studi di Torino: AperTo (Archivio Istituzionale ad Accesso Aperto) |
| Subject Terms: |
BAP1-TPDS; cancer genome; diagnostic; germline variant; immunohistochemistry; melanoma; mesothelioma |
| Description: |
Germline mutations in the tumor suppressor gene BRCA1-associated protein-1 (BAP1) lead to BAP1 tumor predisposition syndrome (BAP1-TPDS), characterized by high susceptibility to several tumor types, chiefly melanoma, mesothelioma, renal cell carcinoma, and basal cell carcinoma. Here, we present the results of our ten-year experience in the molecular diagnosis of BAP1-TPDS, along with a clinical update and cascade genetic testing of previously reported BAP1-TPDS patients and their relatives. Specifically, we sequenced germline DNA samples from 101 individuals with suspected BAP1-TPDS and validated pathogenic variants (PVs) by assessing BAP1 somatic loss in matching tumor specimens. Overall, we identified seven patients (7/101, 6.9%) carrying six different germline BAP1 PVs, including one novel variant. Consistently, cascade testing revealed a total of seven BAP1 PV carriers. In addition, we explored the mutational burden of BAP1-TPDS tumors by targeted next-generation sequencing. Lastly, we found that certain tumors present in PV carriers retain a wild-type BAP1 allele, suggesting a sporadic origin of these tumors or a functional role of heterozygous BAP1 in neoplastic development. Altogether, our findings have important clinical implications for therapeutic response of BAP1-TPDS patients. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/35885614; info:eu-repo/semantics/altIdentifier/wos/WOS:000833091400001; volume:12; issue:7; firstpage:1; lastpage:20; numberofpages:20; journal:DIAGNOSTICS; https://hdl.handle.net/2318/1878603; info:eu-repo/semantics/altIdentifier/scopus/2-s2.0-85137375230 |
| DOI: |
10.3390/diagnostics12071710 |
| Availability: |
https://hdl.handle.net/2318/1878603; https://doi.org/10.3390/diagnostics12071710 |
| Rights: |
info:eu-repo/semantics/openAccess |
| Accession Number: |
edsbas.8EE52708 |
| Database: |
BASE |