| Title: |
Interleukin-27 polymorphisms are associated with inflammatory bowel diseases in a Korean population. |
| Authors: |
Li, CS; Zhang, Q; Lee, KJ; Cho, SW; Lee, KM; Hahm, KB; Choi, SC; Yun, KJ; Chung, HT; Chae, SC |
| Contributors: |
101480; 103828; 조, 성원; 이, 기명 |
| Publication Year: |
2009 |
| Subject Terms: |
Asian Continental Ancestry Group; Case-Control Studies; Colitis; Ulcerative; Crohn Disease; Gene Frequency; Genetic Predisposition to Disease; Haplotypes; Humans; Interleukins; Logistic Models; Odds Ratio; Polymorphism; Single Nucleotide; Republic of Korea; Risk Assessment; Risk Factors |
| Description: |
BACKGROUND AND AIMS: The cytokine interleukin (IL)-27 is composed of two subunits, Epstein-Barr virus-induced gene 3 (EBI3) and p28, and IL-27 is a novel IL-12 family member that mediates between the innate and adaptive immune systems. We previously identified four polymorphisms in the human IL-27 gene and we suggested that the polymorphism of IL-27 is associated with the susceptibility to asthma. IL-27 transcripts are significantly elevated in active Crohn's disease (CD) but not in ulcerative colitis (UC). To determine whether these IL-27 single nucleotide polymorphisms are associated with the susceptibility to inflammatory bowel disease (IBD), the genotype and allelic frequencies of the IL-27 polymorphisms were analyzed between the IBD patients and the healthy controls. METHODS: Genotype analysis of the IL-27 gene was performed by the single-base extension (SBE) method. The haplotype frequencies of IL-27 for multiple loci were estimated using the expectation maximization (EM) algorithm. RESULTS: The genotype frequencies of the g.-964A > G polymorphism in the IBD patients were significantly different from those of the healthy control group (P = 0.001). In both the UC and CD patients, the genotype frequencies of the g.-964A > G polymorphism were also significantly different from the frequencies of the healthy control group (P = 0.009). The frequencies of the AGT and GGT haplotypes were significantly different between the healthy control group and the IBD patient group (P = 0.00004 and 0.021, respectively). CONCLUSION: Our results suggest that the g.-964A > G polymorphism of the IL-27 gene located on the IBD1 locus might be associated with the susceptibility to IBD. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
J008159319; http://repository.ajou.ac.kr/handle/201003/605 |
| DOI: |
10.1111/j.1440-1746.2009.05901.x |
| Availability: |
http://repository.ajou.ac.kr/handle/201003/605; https://doi.org/10.1111/j.1440-1746.2009.05901.x |
| Accession Number: |
edsbas.8FC00533 |
| Database: |
BASE |