| Title: |
A refinedrefined low-dose murine model of Mycobacterium ulcerans infection to assess integrated immune networks in Buruli ulcer pathogenesis |
| Authors: |
Muhi, S; Foo, IJH; Kedzierski, L; Porter, JL; McQuilten, HA; Howden, B; Kedzierska, K; Buultjens, AH; Chua, BY; Stinear, TP |
| Contributors: |
Kline, KA |
| Publisher Information: |
American Society for Microbiology |
| Publication Year: |
2025 |
| Collection: |
The University of Melbourne: Digital Repository |
| Description: |
Mycobacterium ulcerans, the causative agent of Buruli ulcer (BU), has a low infectious dose (70 clinical, immunological, and microbiological parameters. Principal findings included a 100% infection rate even at the lowest inoculum, but with a dose-dependent delay in lesion onset and disease progression for both mouse strains. Bacterial growth kinetics were similar between mouse strains, but there was a difference in immune profiles between mouse strains and between “low” (10 CFU) versus “high” (100 CFU) bacterial challenges. C57BL/6 mice exhibited more robust systemic cellular responses and more rapid lesion onset compared to BALB/c mice. Activated CD8+ T cells and dendritic cells were dominant in responses to low-dose infection in C57BL/6 mice. Murine low-dose M. ulcerans infection models provide confidence for future human Buruli ulcer challenge trials and will inform the development of effective vaccines and therapeutics. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| ISSN: |
2161-2129 |
| Relation: |
https://hdl.handle.net/11343/364124 |
| Availability: |
https://hdl.handle.net/11343/364124 |
| Rights: |
https://creativecommons.org/licenses/by/4.0 ; CC BY |
| Accession Number: |
edsbas.91877AB7 |
| Database: |
BASE |