| Title: |
A Class II-Restricted CD8γ13 T-Cell Clone Protects During Chlamydia muridarum Genital Tract Infection |
| Authors: |
Johnson, Raymond M; Olivares-Strank, Norma; Peng, Gang |
| Contributors: |
National Institute of Allergy and Infectious Diseases; National Institutes of Health |
| Source: |
The Journal of Infectious Diseases ; volume 221, issue 11, page 1895-1906 ; ISSN 0022-1899 1537-6613 |
| Publisher Information: |
Oxford University Press (OUP) |
| Publication Year: |
2020 |
| Description: |
Background The T-cell response to chlamydia genital tract infections in humans and mice is unusual because the majority of antigen-specific CD8 T cells are not class I restricted (referred to here as “unrestricted” or “atypical”). We previously reported that a subset of unrestricted murine chlamydia-specific CD8 T cells had a cytokine polarization pattern that included interferon (IFN)-γ and interleukin (IL)-13. Methods In this study, we investigated the transcriptome of CD8γ13 T cells, comparing them to Tc1 clones using microarray analysis. That study revealed that CD8γ13 polarization included IL-5 in addition to IFN-γ and IL-13. Adoptive transfer studies were performed with Tc1 clones and a CD8γ13 T-cell clone to determine whether either influenced bacterial clearance or immunopathology during Chlamydia muridarum genital tract infections. Results To our surprise, an adoptively transferred CD8γ13 T-cell clone was remarkably proficient at preventing chlamydia immunopathology, whereas the multifunctional Tc1 clone did not enhance clearance or significantly alter immunopathology. Mapping studies with major histocompatibility complex (MHC) class I- and class II-deficient splenocytes showed our previously published chlamydia-specific CD8 T-cell clones are MHC class II restricted. Conclusions The MHC class II-restricted CD8 T cells may play an important role in protection from intracellular pathogens that limit class I antigen presentation or diminish CD4 T-cell numbers or impair their function. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1093/infdis/jiz685 |
| DOI: |
10.1093/infdis/jiz685/32924632/jiz685.pdf |
| Availability: |
https://doi.org/10.1093/infdis/jiz685; http://academic.oup.com/jid/advance-article-pdf/doi/10.1093/infdis/jiz685/32924632/jiz685.pdf; http://academic.oup.com/jid/article-pdf/221/11/1895/33202445/jiz685.pdf |
| Rights: |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
| Accession Number: |
edsbas.91BED460 |
| Database: |
BASE |