| Title: |
Protocol for inspecting blood cell dynamics with a custom ektacytometer-rheometer apparatus |
| Authors: |
Fitzpatrick, T; Simmonds, MJ; McNamee, AP |
| Publisher Information: |
Elsevier BV |
| Publication Year: |
2022 |
| Collection: |
Griffith University: Griffith Research Online |
| Subject Terms: |
Haematology; Biophysics; Biotechnology and bioengineering; Cell Biology; Cell Membrane; Microscopy |
| Description: |
Investigating flowing red blood cell (RBC) morphology and orientation is important for elucidating physiology and disease; existing commercially available products are limited to observing cell populations or single cells. In this protocol, we create a custom apparatus that combines coaxial brightfield microscopy with laser diffractometry to inspect near-real-time deformability, morphology, and orientation of flowing RBCs. There are difficulties associated with building optical systems for biological inspection; however, this protocol provides a suitable framework for developing an “ektacytoscope” for studying blood cells. For complete details on the use and execution of this protocol, please refer to McNamee et al. (2020). ; Full Text |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
STAR Protocols; Fitzpatrick, T; Simmonds, MJ; McNamee, AP, Protocol for inspecting blood cell dynamics with a custom ektacytometer-rheometer apparatus, STAR Protocols, 2022, 3 (2), pp. 101279; https://hdl.handle.net/10072/421833 |
| DOI: |
10.1016/j.xpro.2022.101279 |
| Availability: |
https://hdl.handle.net/10072/421833; https://doi.org/10.1016/j.xpro.2022.101279 |
| Rights: |
http://creativecommons.org/licenses/by-nc-nd/4.0/ ; © 2022 The Author(s). This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). ; open access |
| Accession Number: |
edsbas.91FCCEFE |
| Database: |
BASE |