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Interferon-independent STING enforces epithelial genome-integrity checkpoint to restrain tumor evolution

Title: Interferon-independent STING enforces epithelial genome-integrity checkpoint to restrain tumor evolution
Authors: Xiang, Hang; Macino, Martina; Woitaske-Proske, Clemens; Bodenstein, Nicholas; Bernardes, Joana P.; Tran, Florian; Schmid, Nicole A.; Martini, Gabriela Rios; Wotawa, Felix; Bornhaeuser, Johanna; Kakavand, Nassim; Dyck Dionisi, Oliver; van den Bossche, Silke; Yang, Guang; Wu, Qicong; Kugler, Julia; Welz, Lina; Konukiewitz, Bjorn; Fischer, Julius C.; Peifer, Christian; Schreiber, Stefan; Rosenstiel, Philip; Tschurtschenthaler, Markus; Sanders, Ashley D.; Aden, Konrad
Publisher Information: Cold Spring Harbor Laboratory Press
Publication Year: 2026
Collection: Max-Delbrueck-Center for Molecular Medicine, Berlin: MDC Repository
Subject Terms: Cancer Research; Topic 1: Genes; Cells and Cell-Based Medicine
Description: STING is canonically known for mediating interferon responses to cytosolic DNA, yet its cell-intrinsic role in genome maintenance beyond the immune context is unknown. Here we show that epithelial STING functions as a type I interferon-independent genome-integrity checkpoint. STING loss impairs homologous recombination repair, attenuates ATM-associated damage signaling, elevates CDK1 activity, and causes chromosomal instability revealed by single-cell Strand-seq, culminating in spontaneous intestinal adenocarcinoma. These defects arise before tumor formation and confer selective vulnerability to CDK inhibition in tumor organoids and human colorectal cancer cells. Our findings identify STING as a cell-autonomous guardian of epithelial genome stability that restrains chromosomal instability-driven tumor evolution beyond its canonical immune function.
Document Type: report
File Description: application/pdf
Language: English
Relation: https://edoc.mdc-berlin.de/id/eprint/26199/1/26199oa.pdf; Interferon-independent STING enforces epithelial genome-integrity checkpoint to restrain tumor evolution. Xiang, Hang, Macino, Martina, Woitaske-Proske, Clemens, Bodenstein, Nicholas, Bernardes, Joana P., Tran, Florian, Schmid, Nicole A., Martini, Gabriela Rios, Wotawa, Felix, Bornhaeuser, Johanna, Kakavand, Nassim, Dyck Dionisi, Oliver, van den Bossche, Silke, Yang, Guang, Wu, Qicong, Kugler, Julia, Welz, Lina, Konukiewitz, Bjorn, Fischer, Julius C., Peifer, Christian, Schreiber, Stefan, Rosenstiel, Philip, Tschurtschenthaler, Markus, Sanders, Ashley D. and Aden, Konrad bioRxiv : 2026.02.17.706442v2. 25 March 2026; bioRxiv:2026.02.17.706442v2; https://doi.org/10.64898/2026.02.17.706442
DOI: 10.64898/2026.02.17.706442
Availability: https://edoc.mdc-berlin.de/id/eprint/26199/; https://edoc.mdc-berlin.de/26199/; https://doi.org/10.64898/2026.02.17.706442
Rights: cc_by_4
Accession Number: edsbas.9256D500
Database: BASE