| Title: |
Oxidative Stress Evaluation in Ischemia Reperfusion Models: Characteristics, Limits and Perspectives |
| Authors: |
Chazelas, Pauline; Steichen, Clara; Favreau, Frédéric; Trouillas, Patrick; Hannaert, Patrick; Thuillier, Raphaël; Giraud, Sébastien; Hauet, Thierry; Guillard, Jérôme |
| Contributors: |
Maintenance Myélinique et Neuropathies Périphériques (MMNP); Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST); Université de Limoges (UNILIM)-Université de Limoges (UNILIM); Service de Biochimie et Génétique Moléculaire CHU Limoges; CHU Limoges; Ischémie Reperfusion en Transplantation d’Organes Mécanismes et Innovations Thérapeutiques U 1082 (IRTOMIT Poitiers ); Université de Poitiers = University of Poitiers (UP)-Institut National de la Santé et de la Recherche Médicale (INSERM); Ciblage individuel et prévention des risques de traitements immunosupresseurs et de la transplantation (IPPRITT); CHU Limoges-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST); Regional Centre of Advanced Technologies and Materials Olomouc (RCPTM); Centre hospitalier universitaire de Poitiers = Poitiers University Hospital (CHU de Poitiers La Milétrie ); Fédération Hospitalo-universitaire SUrvival oPtimization in ORgan Transplantation (FHU SUPORT); Université de Poitiers = University of Poitiers (UP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Université de Poitiers = University of Poitiers (UP)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Anti-infectieux : supports moléculaires des résistances et innovations thérapeutiques (RESINFIT); Université de Limoges (UNILIM)-Université de Limoges (UNILIM)-CHU Limoges-Institut National de la Santé et de la Recherche Médicale (INSERM)-Institut Génomique, Environnement, Immunité, Santé, Thérapeutique (GEIST); Université de Limoges (UNILIM)-Université de Limoges (UNILIM)-Centre hospitalier universitaire de Poitiers = Poitiers University Hospital (CHU de Poitiers La Milétrie )-Ciblage individuel et prévention des risques de traitements immunosupresseurs et de la transplantation (IPPRITT); Université de Limoges (UNILIM)-Université de Limoges (UNILIM)-Institut National de la Santé et de la Recherche Médicale (INSERM)-Transplantation, Immunologie, Inflammation Tours (T2i); Université de Tours (UT)-Université de Tours (UT); MOPICT – MOdélisation Préclinique, Innovation Chirurgicale et Technologique Plateforme labellisée IBiSA, tutelle INRAE (MOPICT); Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE); Institut de chimie des milieux et matériaux de Poitiers UMR 7285 (IC2MP Poitiers ); Université de Poitiers = University of Poitiers (UP)-Institut de Chimie - CNRS Chimie (INC-CNRS)-Centre National de la Recherche Scientifique (CNRS) |
| Source: |
ISSN: 1661-6596. |
| Publisher Information: |
CCSD; MDPI |
| Publication Year: |
2021 |
| Collection: |
Université François-Rabelais de Tours: HAL |
| Subject Terms: |
ischemia-reperfusion injury; antioxidant factors; Reactive Oxygen Species (ROS); oxidative stress; animal models; organoids; molecular modeling models; [SDV.MHEP]Life Sciences [q-bio]/Human health and pathology |
| Description: |
International audience ; Ischemia reperfusion injury is a complex process consisting of a seemingly chaotic but actually organized and compartmentalized shutdown of cell function, of which oxidative stress is a key component. Studying oxidative stress, which results in an imbalance between reactive oxygen species (ROS) production and antioxidant defense activity, is a multi-faceted issue, particularly considering the double function of ROS, assuming roles as physiological intracellular signals and as mediators of cellular component damage. Herein, we propose a comprehensive overview of the tools available to explore oxidative stress, particularly in the study of ischemia reperfusion. Applying chemistry as well as biology, we present the different models currently developed to study oxidative stress, spanning the vitro and the silico, discussing the advantages and the drawbacks of each set-up, including the issues relating to the use of in vitro hypoxia as a surrogate for ischemia. Having identified the limitations of historical models, we shall study new paradigms, including the use of stem cell-derived organoids, as a bridge between the in vitro and the in vivo comprising 3D intercellular interactions in vivo and versatile pathway investigations in vitro. We shall conclude this review by distancing ourselves from “wet” biology and reviewing the in silico, computer-based, mathematical modeling, and numerical simulation options: (a) molecular modeling with quantum chemistry and molecular dynamic algorithms, which facilitates the study of molecule-to-molecule interactions, and the integration of a compound in a dynamic environment (the plasma membrane.); (b) integrative systemic models, which can include many facets of complex mechanisms such as oxidative stress or ischemia reperfusion and help to formulate integrated predictions and to enhance understanding of dynamic interaction between pathways. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.3390/ijms22052366 |
| Availability: |
https://hal.science/hal-03224524; https://hal.science/hal-03224524v1/document; https://hal.science/hal-03224524v1/file/ijms-22-02366-v3.pdf; https://doi.org/10.3390/ijms22052366 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.925FFD48 |
| Database: |
BASE |