| Title: |
In Vitro Characterization of Centella asiatica Extracellular Vesicles and Their Skin Repair Effects in a UVB-Irradiated Mouse Model |
| Authors: |
Tsong-Min Chang; Chung-Chin Wu; Huey-Chun Huang; Shr-Shiuan Wang; Ching-Hua Chuang; Pei-Lun Kao; Wei-Hsuan Tang; Luke Tzu-Chi Liu; Wei-Yin Qiu; Ivona Percec; Charles Chen; Tsun-Yung Kuo |
| Source: |
International Journal of Molecular Sciences ; Volume 26 ; Issue 18 ; Pages: 8982 |
| Publisher Information: |
Multidisciplinary Digital Publishing Institute |
| Publication Year: |
2025 |
| Collection: |
MDPI Open Access Publishing |
| Subject Terms: |
Centella asiatica; skin care; extracellular vesicles; antioxidants; cosmetics; anti-inflammatory; skin whitening; UV damage; photoaging |
| Description: |
This study characterized extracellular vesicles (EVs) isolated from medicinal herb Centella asiatica tissue culture and investigated their therapeutic properties using in vitro assays and a ultraviolet (UV)-induced damage mouse model. EVs were isolated from C. asiatica tissue culture and characterized by nanoparticle tracking analysis, and cytotoxicity, antioxidant, anti-melanin, and anti-inflammation properties were evaluated by in vitro assays. C. asiatica EVs were found to contain high levels of polyphenols and mitigate hydrogen peroxide-induced intracellular reactive oxygen species (ROS). The EVs were further able to reduce intracellular melanin content and tyrosinase activity. They exhibited anti-inflammatory effects by downregulating the expression of pro-inflammatory genes, COX2, as well as nitric oxide production. In the UV-induced photodamage mouse model, gels with or without EVs were applied to the UV-damaged site, skin appearance was observed daily, and skin histopathology was analyzed on day 7. In mice with UV-induced skin damage, the daily application of C. asiatica EV gel reduced skin epidermis thickness and inflammation compared to UV-only or blank gel at seven days after UV irradiation. The beneficial effects of C. asiatica EVs on skin quality warrant further studies as promising agents in skin care applications. |
| Document Type: |
text |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
Bioactives and Nutraceuticals; https://dx.doi.org/10.3390/ijms26188982 |
| DOI: |
10.3390/ijms26188982 |
| Availability: |
https://doi.org/10.3390/ijms26188982 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.9261F641 |
| Database: |
BASE |