| Title: |
Analysis of retrotransposon subfamily DNA methylation reveals novel early epigenetic changes in chronic lymphocytic leukemia. |
| Authors: |
Barrow, Timothy M; Wong Doo, Nicole; Milne, Roger L; Giles, Graham G; Willmore, Elaine; Strathdee, Gordon; Byun, Hyang-Min |
| Publisher Information: |
Ferrata Storti Foundation |
| Publication Year: |
2021 |
| Collection: |
University of Essex Research Repository |
| Description: |
Retrotransposons such as LINE-1 and Alu comprise >25% of the human genome. While global hypomethylation of these elements has been widely reported in solid tumours, their epigenetic dysregulation is yet to be characterised in chronic lymphocytic leukaemia, and there has been scant consideration of their evolutionary history that mediates sensitivity to hypomethylation. Here, we developed an approach for locus- and evolutionary subfamily-specific analysis of retrotransposons using the Illumina Infinium Human Methylation 450K microarray platform, which we applied to publicly-available datasets from chronic lymphocytic leukaemia and other haematological malignancies. We identified 9,797 microarray probes mapping to 117 LINE-1 subfamilies and 13,130 mapping to 37 Alu subfamilies. Of these, 10,782 were differentially methylated (PFDR90% patients) but not observed in healthy individuals or other leukaemias, and was detectable in blood samples taken prior to chronic lymphocytic leukaemia diagnosis in 9 of 82 individuals from the Melbourne Collaborative Cohort Study. Our results demonstrate differential methylation of retrotransposons in chronic lymphocytic leukaemia by their evolutionary heritage that modulates expression of proximal genes. |
| Document Type: |
article in journal/newspaper |
| File Description: |
text |
| Language: |
English |
| Relation: |
https://repository.essex.ac.uk/34489/1/Analysis%20of%20retrotransposon%20subfamily%20DNA%20methylation%20reveals%20novel%20early%20epigenetic%20changes%20in%20chronic%20lymphocytic%20leukemia.pdf; Barrow, Timothy M and Wong Doo, Nicole and Milne, Roger L and Giles, Graham G and Willmore, Elaine and Strathdee, Gordon and Byun, Hyang-Min (2021) Analysis of retrotransposon subfamily DNA methylation reveals novel early epigenetic changes in chronic lymphocytic leukemia. Haematologica, 106 (1). pp. 98-110. DOI https://doi.org/10.3324/haematol.2019.228478 |
| DOI: |
10.3324/haematol.2019.228478 |
| Availability: |
https://repository.essex.ac.uk/34489/; https://doi.org/10.3324/haematol.2019.228478; https://repository.essex.ac.uk/34489/1/Analysis%20of%20retrotransposon%20subfamily%20DNA%20methylation%20reveals%20novel%20early%20epigenetic%20changes%20in%20chronic%20lymphocytic%20leukemia.pdf |
| Rights: |
cc_by_nc_4 |
| Accession Number: |
edsbas.940D2B14 |
| Database: |
BASE |