| Title: |
Microhomologies are prevalent at Cas9-induced larger deletions |
| Authors: |
Owens, Dominic D G; Caulder, Adam; Frontera, Vincent; Harman, Joe R; Allan, Alasdair J; Bucakci, Akin; Greder, Lucas; Codner, Gemma F; Hublitz, Philip; McHugh, Peter J; Teboul, Lydia; de Bruijn, Marella F T R |
| Contributors: |
National Institute for Health; NIHR Oxford BRC and John Fell Fund; U.S. Environmental Protection Agency; WIMM Strategic Alliance awards |
| Source: |
Nucleic Acids Research ; volume 47, issue 14, page 7402-7417 ; ISSN 0305-1048 1362-4962 |
| Publisher Information: |
Oxford University Press (OUP) |
| Publication Year: |
2019 |
| Description: |
The CRISPR system is widely used in genome editing for biomedical research. Here, using either dual paired Cas9D10A nickases or paired Cas9 nuclease we characterize unintended larger deletions at on-target sites that frequently evade common genotyping practices. We found that unintended larger deletions are prevalent at multiple distinct loci on different chromosomes, in cultured cells and mouse embryos alike. We observed a high frequency of microhomologies at larger deletion breakpoint junctions, suggesting the involvement of microhomology-mediated end joining in their generation. In populations of edited cells, the distribution of larger deletion sizes is dependent on proximity to sgRNAs and cannot be predicted by microhomology sequences alone. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1093/nar/gkz459 |
| DOI: |
10.1093/nar/gkz459/28704124/gkz459.pdf |
| Availability: |
https://doi.org/10.1093/nar/gkz459; http://academic.oup.com/nar/advance-article-pdf/doi/10.1093/nar/gkz459/28704124/gkz459.pdf; http://academic.oup.com/nar/article-pdf/47/14/7402/29184886/gkz459.pdf |
| Rights: |
http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.9479D971 |
| Database: |
BASE |