| Title: |
A specific 31-nucleotide domain of U1 RNA directly interacts with the 70K small nuclear ribonucleoprotein component. |
| Authors: |
Query, C C; Bentley, R C; Keene, J D |
| Source: |
Molecular and Cellular Biology ; volume 9, issue 11, page 4872-4881 ; ISSN 0270-7306 1098-5549 |
| Publisher Information: |
Informa UK Limited |
| Publication Year: |
1989 |
| Description: |
We have defined the nucleotide sequence of a protein-binding domain within U1 RNA that specifically recognizes and binds both to a U1 small nuclear ribonucleoprotein component (the 70K protein) and to the previously defined RNA-binding domain of the 70K protein. We have investigated direct interactions between purified U1 RNA and 70K protein by reconstitution in vitro. Thirty-one nucleotides of U1 RNA, corresponding to stem-loop I, were required for this interaction. Nucleotides at the 5' end of U1 RNA that are involved in base pairing with the 5' splice site of pre-mRNA were not required for binding. In contrast to other reports, these findings demonstrate that a specific domain of U1 RNA can bind directly to the 70K protein independently of any other snRNP-associated proteins. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1128/mcb.9.11.4872 |
| DOI: |
10.1128/MCB.9.11.4872 |
| Availability: |
https://doi.org/10.1128/mcb.9.11.4872; https://syndication.highwire.org/content/doi/10.1128/MCB.9.11.4872 |
| Accession Number: |
edsbas.94B6E407 |
| Database: |
BASE |