| Title: |
Electrospray ion‐trap multistage mass spectrometry for characterisation of co‐monomer compositional distribution of bacterial poly(3‐hydroxybutyrate‐co‐3‐hydroxyhexanoate) at the molecular level |
| Authors: |
Adamus, Grażyna; Sikorska, Wanda; Kowalczuk, Marek; Noda, Isao; Satkowski, Michael M. |
| Source: |
Rapid Communications in Mass Spectrometry ; volume 17, issue 20, page 2260-2266 ; ISSN 0951-4198 1097-0231 |
| Publisher Information: |
Wiley |
| Publication Year: |
2003 |
| Collection: |
Wiley Online Library (Open Access Articles via Crossref) |
| Description: |
We report an electrospray ionisation multistage mass spectrometry (ESI‐MS n ) method that utilises molecular mass information for determination of sequence distribution and chemical structure of mass‐selected macromolecules of poly(3‐hydroxybutyrate‐ co ‐3‐hydroxyhexanoate) biopolyester, PHBH. On the basis of ESI‐MS n studies of PHBH oligomers obtained by partial alkaline depolymerisation of natural PHBH containing 13–14 mol% of hydroxyhexanoate (HH) units, the microstructure of this bacterial copolyester was assessed up to the level of 28 repeat units. The subtle structural details of the PHBH were evaluated based on sequencing of individual macromolecular ions thus showing the utility of this technique for the analysis of biological copolyester macromolecules. It was confirmed that both HH and hydroxybutyrate (HB) units of the PHBH copolymer are randomly distributed. Copyright © 2003 John Wiley & Sons, Ltd. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1002/rcm.1190 |
| Availability: |
https://doi.org/10.1002/rcm.1190; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Frcm.1190; https://onlinelibrary.wiley.com/doi/full/10.1002/rcm.1190 |
| Rights: |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
| Accession Number: |
edsbas.9578CC3A |
| Database: |
BASE |