| Title: |
Fine-tuning of T cell development by the CD3{gamma} di-leucine-based TCR sorting motif |
| Authors: |
Lauritsen, Jens Peter H.; Boding, Lasse; Buus, Terkild B.; Kongsbak, Martin; Levring, Trine B.; Rode, Anna K.O.; Bonefeld, Charlotte Menné; Geisler, Carsten |
| Publisher Information: |
Oxford University Press |
| Publication Year: |
2015 |
| Collection: |
HighWire Press (Stanford University) |
| Subject Terms: |
Original Research |
| Description: |
The CD3γ di-leucine-based (diL) receptor-sorting motif plays a central role in TCR down-regulation and in clonal expansion of virus-specific T cells. However, the role of the CD3γ diL motif in T cell development is not known. In this study, we show that protein kinase C-induced TCR down-regulation is abolished in thymocytes from CD3γLLAA mice with a mutated CD3γ diL motif, and that CD3γLLAA mice have reduced numbers of thymocytes compared to aged-matched wild-type mice. We found that early thymocyte development at the β-selection checkpoint is impaired resulting in reduced numbers of double negative (DN) 4 cells in CD3γLLAA mice. This was not caused by reduced proliferation but most probably by increased down-regulation of the anti-apoptotic molecule Bcl-2 causing enhanced apoptosis during the transition from the DN3 to the DN4 stage. In contrast, proliferation of immature CD8 single positive (ISP) thymocytes was increased resulting in normal numbers of ISP in CD3γLLAA mice. Despite the normal numbers of ISP, CD3γLLAA mice had reduced numbers of double positive and single positive thymocytes indicating that the CD3γ diL motif also affected later stages of T cell development. In accordance, we found that positive and negative selection, differentiation towards CD4 and CD8 single positive T cells and the development of non-conventional T cells were affected in CD3γLLAA mice. In conclusion, our study identifies an important role of the CD3γ diL motif in T cell development most probably mediated by its fine-tuning of pre-TCR and TCR expression, down-regulation and signaling. |
| Document Type: |
text |
| File Description: |
text/html |
| Language: |
English |
| Relation: |
http://intimm.oxfordjournals.org/cgi/content/short/dxv022v1; http://dx.doi.org/10.1093/intimm/dxv022 |
| DOI: |
10.1093/intimm/dxv022 |
| Availability: |
http://intimm.oxfordjournals.org/cgi/content/short/dxv022v1; https://doi.org/10.1093/intimm/dxv022 |
| Rights: |
Copyright (C) 2015, The Japanese Society for Immunology |
| Accession Number: |
edsbas.959C9EE |
| Database: |
BASE |