| Title: |
Solution structure of CCL19 and identification of overlapping CCR7 and PSGL-1 binding sites |
| Authors: |
Veldkamp, Christopher; Kiermaier, Eva; Gabel Eissens, Skylar; Gillitzer, Miranda; Lippner, David; Disilvio, Frank; Mueller, Casey; Wantuch, Paeton; Chaffee, Gary; Famiglietti, Michael; Zgoba, Danielle; Bailey, Asha; Bah, Yaya; Engebretson, Samantha; Graupner, David; Lackner, Emily; Larosa, Vincent; Medeiros, Tysha; Olson, Michael; Phillips, Andrew; Pyles, Harley; Richard, Amanda; Schoeller, Scott; Touzeau, Boris; Williams, Larry; Sixt, Michael K; Peterson, Francis |
| Source: |
Veldkamp C, Kiermaier E, Gabel Eissens S, et al. Solution structure of CCL19 and identification of overlapping CCR7 and PSGL-1 binding sites. Biochemistry . 2015;54(27):4163-4166. doi: 10.1021/acs.biochem.5b00560 |
| Publisher Information: |
American Chemical Society |
| Publication Year: |
2015 |
| Collection: |
IST Austria Research Explorer (Institute of Science and Technology) |
| Description: |
CCL19 and CCL21 are chemokines involved in the trafficking of immune cells, particularly within the lymphatic system, through activation of CCR7. Concurrent expression of PSGL-1 and CCR7 in naive T-cells enhances recruitment of these cells to secondary lymphoid organs by CCL19 and CCL21. Here the solution structure of CCL19 is reported. It contains a canonical chemokine domain. Chemical shift mapping shows the N-termini of PSGL-1 and CCR7 have overlapping binding sites for CCL19 and binding is competitive. Implications for the mechanism of PSGL-1's enhancement of resting T-cell recruitment are discussed. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/wos/000358105100001; info:eu-repo/semantics/altIdentifier/pmid/26115234 |
| Availability: |
https://research-explorer.ista.ac.at/record/1618 |
| Rights: |
info:eu-repo/semantics/openAccess |
| Accession Number: |
edsbas.967FD6EB |
| Database: |
BASE |