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Discovery of 4,6-disubstituted pyrimidines as potent inhibitors of the heat shock factor 1 (HSF1) stress pathway and CDK9.

Title: Discovery of 4,6-disubstituted pyrimidines as potent inhibitors of the heat shock factor 1 (HSF1) stress pathway and CDK9.
Authors: Rye, CS; Chessum, NEA; Lamont, S; Pike, KG; Faulder, P; Demeritt, J; Kemmitt, P; Tucker, J; Zani, L; Cheeseman, MD; Isaac, R; Goodwin, L; Boros, J; Raynaud, F; Hayes, A; Henley, AT; de Billy, E; Lynch, CJ; Sharp, SY; Te Poele, R; Fee, LO; Foote, KM; Green, S; Workman, P; Jones, K
Contributors: Cheeseman, Matthew; Raynaud, Florence; Hayes, Andrew; Workman, Paul; Jones, Keith
Publisher Information: ROYAL SOC CHEMISTRY
Publication Year: 2016
Collection: The Institute of Cancer Research (ICR): Publications Repository
Description: Heat shock factor 1 (HSF1) is a transcription factor that plays key roles in cancer, including providing a mechanism for cell survival under proteotoxic stress. Therefore, inhibition of the HSF1-stress pathway represents an exciting new opportunity in cancer treatment. We employed an unbiased phenotypic screen to discover inhibitors of the HSF1-stress pathway. Using this approach we identified an initial hit (1) based on a 4,6-pyrimidine scaffold (2.00 μM). Optimisation of cellular SAR led to an inhibitor with improved potency (25, 15 nM) in the HSF1 phenotypic assay. The 4,6-pyrimidine 25 was also shown to have high potency against the CDK9 enzyme (3 nM).
Document Type: article in journal/newspaper
File Description: Print-Electronic; 1586; application/octet-stream
Language: English
ISSN: 2040-2511; 2040-2503
Relation: MedChemComm, 2016, 7 (8), pp. 1580 - 1586; https://repository.icr.ac.uk/handle/internal/120
DOI: 10.1039/c6md00159a
Availability: https://doi.org/10.1039/c6md00159a; https://repository.icr.ac.uk/handle/internal/120
Rights: https://creativecommons.org/licenses/by/4.0
Accession Number: edsbas.968FB104
Database: BASE