| Title: |
Human Milk Antibodies against S1 and S2 Subunits from SARS-CoV-2, HCoV-OC43, and HCoV-229E in Mothers with a Confirmed COVID-19 PCR, Viral SYMPTOMS, and Unexposed Mothers |
| Authors: |
Demers-Mathieu, Veronique; DaPra, Ciera; Mathijssen, Gabrielle; Sela, David A.; Järvinen, Kirsi M.; Seppo, Antti; Fels, Shawn; Medo, Elena |
| Source: |
Food Science Department Faculty Publication Series |
| Publisher Information: |
ScholarWorks@UMass Amherst |
| Publication Year: |
2021 |
| Collection: |
University of Massachusetts: ScholarWorks@UMass Amherst |
| Subject Terms: |
breast milk; passive immunity; immunoglobulins; infectious diseases; breastfeeding; polyreactive antibody; β-coronaviruses; α-coronaviruses; neonates |
| Description: |
Background: Preexisting immunity to SARS-CoV-2 could be related to cross-reactive antibodies to common human-coronaviruses (HCoVs). This study aimed to evaluate whether human milk antibodies against to S1 and S2 subunits SARS-CoV-2 are cross-reactive to S1 and S2 subunits HCoV-OC43 and HCoV-229E in mothers with a confirmed COVID-19 PCR test, in mothers with previous viral symptoms during COVID-19 pandemic, and in unexposed mothers; Methods: The levels of secretory IgA (SIgA)/IgA, secretory IgM (SIgM)/IgM, and IgG specific to S1 and S2 SARS-CoV-2, and reactive to S1 + S2 HCoV-OC43, and HCoV-229E were measured in milk from 7 mothers with a confirmed COVID-19 PCR test, 20 mothers with viral symptoms, and unexposed mothers (6 Ctl1-2018 and 16 Ctl2-2018) using ELISA; Results: The S2 SARS-CoV-2 IgG levels were higher in the COVID-19 PCR (p = 0.014) and viral symptom (p = 0.040) groups than in the Ctl1-2018 group. We detected a higher number of positive correlations between the antigens and secretory antibodies in the COVID-19 PCR group than in the viral symptom and Ctl-2018 groups. S1 + S2 HCoV-OC43-reactive IgG was higher in the COVID-19 group than in the control group (p = 0.002) but did not differ for the other antibodies; Conclusions: Mothers with a confirmed COVID-19 PCR and mothers with previous viral symptoms had preexisting human milk antibodies against S2 subunit SARS-CoV-2. Human milk IgG were more specific to S2 subunit SARS-CoV-2 than other antibodies, whereas SIgA and SIgM were polyreactive and cross-reactive to S1 or S2 subunit SARS-CoV-2. |
| Document Type: |
text |
| File Description: |
application/pdf |
| Language: |
unknown |
| Relation: |
https://scholarworks.umass.edu/foodsci_faculty_pubs/20; https://scholarworks.umass.edu/context/foodsci_faculty_pubs/article/1020/viewcontent/ijms_22_01749_v2.pdf |
| DOI: |
10.3390/ijms22041749 |
| Availability: |
https://scholarworks.umass.edu/foodsci_faculty_pubs/20; https://doi.org/10.3390/ijms22041749; https://scholarworks.umass.edu/context/foodsci_faculty_pubs/article/1020/viewcontent/ijms_22_01749_v2.pdf |
| Rights: |
http://creativecommons.org/licenses/by/4.0/ |
| Accession Number: |
edsbas.9850E8AD |
| Database: |
BASE |