| Title: |
PASTA repeats of the protein kinase StkP interconnect cell constriction and separation of Streptococcus pneumoniae |
| Authors: |
Zucchini, L.; Mercy, C.; Garcia, Ps.; Cluzel, C.; Gueguen-Chaignon, V.; Galisson, F.; Freton, C.; Guiral, S.; Brochier-Armanet, Céline; Gouet, P.; Grangeasse, C. |
| Contributors: |
Bioinformatique, phylogénie et génomique évolutive LBBE (BPGE); Département PEGASE LBBE (PEGASE); Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE); Université Claude Bernard Lyon 1 (UCBL); Université de Lyon-Université de Lyon-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL); Université de Lyon-Université de Lyon-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS)-Laboratoire de Biométrie et Biologie Evolutive - UMR 5558 (LBBE); Université de Lyon-Université de Lyon-VetAgro Sup - Institut national d'enseignement supérieur et de recherche en alimentation, santé animale, sciences agronomiques et de l'environnement (VAS)-Centre National de la Recherche Scientifique (CNRS); Région Auvergne-Rhône-Alpes (financial support for L.Z., C.M. and P.S.G.) and Bettencourt Schueller Foundation.; ANR-12-BSV3-0008,PiBaKi,Rôle cellulaire et mode d'action de deux protéine-kinases centrales chez les bactéries(2012); ANR-15-CE32-0001,Map-CellDiv,MapZ: caractérisation d'un nouveau mécanisme de régulation de la division cellulaire bactérienne(2015) |
| Source: |
ISSN: 2058-5276. |
| Publisher Information: |
CCSD; Nature Publishing Group |
| Publication Year: |
2018 |
| Collection: |
HAL Lyon 1 (University Claude Bernard Lyon 1) |
| Subject Terms: |
Bacterial structural biology; Cellular microbiology; Bacterial physiology; Bacterial genes; [SDV]Life Sciences [q-bio] |
| Description: |
International audience ; Eukaryotic-like serine/threonine kinases (eSTKs) with extracellular PASTA repeats are key membrane regulators of bacterial cell division. How PASTA repeats govern eSTK activation and function remains elusive. Using evolution- and structural-guided approaches combined with cell imaging, we disentangle the role of each PASTA repeat of the eSTK StkP from Streptococcus pneumoniae. While the three membrane-proximal PASTA repeats behave as interchangeable modules required for the activation of StkP independently of cell wall binding, they also control the septal cell wall thickness. In contrast, the fourth and membrane-distal PASTA repeat directs StkP localization at the division septum and encompasses a specific motif that is critical for final cell separation through interaction with the cell wall hydrolase LytB. We propose a model in which the extracellular four-PASTA domain of StkP plays a dual function in interconnecting the phosphorylation of StkP endogenous targets along with septal cell wall remodelling to allow cell division of the pneumococcus. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/29203882; PUBMED: 29203882 |
| DOI: |
10.1038/s41564-017-0069-3 |
| Availability: |
https://hal.science/hal-01887855; https://doi.org/10.1038/s41564-017-0069-3 |
| Accession Number: |
edsbas.98DB90AE |
| Database: |
BASE |