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Mixing Ratio and Packaging Amount Synergistically Improved Antioxidant Properties of Baby Lettuce (Lactuca sativa L.) and Spinach (Spinacia oleracea L.) Mixes

Title: Mixing Ratio and Packaging Amount Synergistically Improved Antioxidant Properties of Baby Lettuce (Lactuca sativa L.) and Spinach (Spinacia oleracea L.) Mixes
Authors: Lijuan Zhan; Cosimo M. Profico; Giuseppe Pignata; Manuela Casale; Han Gao; Marco Devecchi; Silvana Nicola
Source: Foods ; Volume 15 ; Issue 3 ; Pages: 499
Publisher Information: Multidisciplinary Digital Publishing Institute
Publication Year: 2026
Collection: MDPI Open Access Publishing
Subject Terms: fresh-cut vegetables; packaging method; food quality; antioxidants; modified atmosphere packaging
Subject Geographic: agris
Description: Fresh baby leaves are commercially marketed in various mixing ratios and packaging amounts, creating very distinct microenvironmental conditions that significantly affect the postharvest quality of the fresh product. This study investigated the synergistic effect of mixing ratio (50LB, 50% lettuce + 50% spinach; 75LB, 75% lettuce + 25% spinach; 100LB, 100% lettuce) and packaging amount (125F, 125 g; 250F, 250 g) on the antioxidant qualities of baby lettuce and spinach mixes during 9 days of storage at 4 °C. The results showed that 50LB × 250F inhibited the degradation of chlorophyll and carotenoids and preserved 28% higher total antioxidant capacity (TAC), 43% higher total phenolic compounds (TPC), and 20% higher vitamin C (Vit.C) than the mean values of all samples, resulting in 0.8% lower O2 and 14.7% higher CO2 levels at the end of storage. TPC, Vit.C, and carotenoids were the main contributors to TAC, with strong correlations (p < 0.001). The total bacterial (TB) and yeast + mold (Y + M) counts were only affected by the mixing ratios, with TB increasing by only 1 Log10 cfu g−1 FW, and Y + M remaining within the same order of magnitude over time. After 9 days of storage, the leaves were still fresh and marketable. This study not only provides a practical strategy for the fresh-cut industry to enhance product quality but also underscores the significance of multifactorial synergism in salad mix packaging.
Document Type: text
File Description: application/pdf
Language: English
Relation: Food Packaging and Preservation; https://dx.doi.org/10.3390/foods15030499
DOI: 10.3390/foods15030499
Availability: https://doi.org/10.3390/foods15030499
Rights: https://creativecommons.org/licenses/by/4.0/
Accession Number: edsbas.993CD51F
Database: BASE