| Title: |
Transcriptional regulation of Zic3 by heterodimeric AP-1(c-Jun/c-Fos) during Xenopus development |
| Authors: |
Lee, SY; Lee, FS; Moon, JS; Kim, JI; Park, JB; Lee, JY; Park, MJ; Kim, J |
| Contributors: |
Kim, JI |
| Publisher Information: |
Springer Nature |
| Publication Year: |
2025 |
| Collection: |
Seoul National University: S-Space |
| Subject Terms: |
NEURAL CREST DEVELOPMENT; FIBROBLAST-GROWTH-FACTOR; C-JUN; MESODERM INDUCTION; AP-1; EMBRYOS; GENE; CELLS; BMP-4; TRANSFORMATION; activin; AP-1(c-Jun &; c-fos); Xenopus development; Zic3 |
| Description: |
The heterodimeric c,-Jun/c-Fos, an activator protein-1 (AP-1) has been implicated in mesoderm induction (Dong et al., 1996; Kim et al., 1998) whereas the homodimer of c-Jun was reported to be involved in neural inhibition during the early development of Xenopus embryos. During the early vertebrate development AP-1 involvement in the neural induction is still not clearly understood. We report here that AP-1 has a role in Zic3 expression, a critical proneural gene and a primary regulator of neural and neural crest development (Nakata et al., 1997; Nakata et al., 1998). AP-1 was able to induce the Zic3 gene in a dose dependent manner but other homo-or hetero-dimeric proteins, such as. c-Jun/c-Jun, JunD/FosB or JunD/Fra-1 were not. The inhibition of AP-1 activity using morpholino antisenses of c-jun mRNAs blocked the Zic3 expression induced by activin. In addition, co-injection of c-jun mRNA rescued the down-regulated Zic3 expression. The promoter region of isolated Zic3 genomic DNA was found to possess several consensus-binding site of AP-1. Thus, in the functional assays, AP-1 could increase promoter activity of Zic3 gene. These findings suggest that proneural gene, Zic3 may be regulated by heterodimeric AP-1(c-Jun/c-Fos) and it may have a role in activin signaling for the regulation of neural specific gene, Zic3. ; Y ; 1 |
| Document Type: |
article in journal/newspaper |
| Language: |
unknown |
| Relation: |
https://hdl.handle.net/10371/225310; 000225359200009; 240478; ART001100152 |
| DOI: |
10.1038/emm.2004.59 |
| Availability: |
https://hdl.handle.net/10371/225310; https://doi.org/10.1038/emm.2004.59 |
| Accession Number: |
edsbas.995ED611 |
| Database: |
BASE |