| Title: |
Rapidly Correcting Frameshift Mutations in the Mycobacterium tuberculosis orn Gene Produce Reversible Ethambutol Resistance and Small-Colony-Variant Morphology |
| Authors: |
Safi, Hassan; Lingaraju, Subramanya; Ma, Shuyi; Husain, Seema; Hoque, Mainul; Soteropoulos, Patricia; Rustad, Tige; Sherman, David R.; Alland, David |
| Contributors: |
HHS | NIH | National Institute of Allergy and Infectious Diseases |
| Source: |
Antimicrobial Agents and Chemotherapy ; volume 64, issue 9 ; ISSN 0066-4804 1098-6596 |
| Publisher Information: |
American Society for Microbiology |
| Publication Year: |
2020 |
| Description: |
We have identified a previously unknown mechanism of reversible high-level ethambutol (EMB) resistance in Mycobacterium tuberculosis that is caused by a reversible frameshift mutation in the M. tuberculosis orn gene. A frameshift mutation in orn produces the small-colony-variant (SCV) phenotype, but this mutation does not change the MICs of any drug for wild-type M. tuberculosis . However, the same orn mutation in a low-level EMB-resistant double embB-aftA mutant (MIC = 8 μg/ml) produces an SCV with an EMB MIC of 32 μg/ml. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1128/aac.00213-20 |
| DOI: |
10.1128/AAC.00213-20 |
| Availability: |
https://doi.org/10.1128/aac.00213-20; https://journals.asm.org/doi/pdf/10.1128/AAC.00213-20 |
| Rights: |
https://journals.asm.org/non-commercial-tdm-license |
| Accession Number: |
edsbas.999262B7 |
| Database: |
BASE |