| Title: |
Tissue expression and actin binding of a novel N-terminal utrophin isoform |
| Authors: |
Zuellig, R A; Bornhauser, B C; Amstutz, R; Constantin, B; Schaub, M C |
| Source: |
Zuellig, R A; Bornhauser, B C; Amstutz, R; Constantin, B; Schaub, M C (2011). Tissue expression and actin binding of a novel N-terminal utrophin isoform. Journal of Biomedicine and Biotechnology, 2011:904547. |
| Publisher Information: |
Hindawi Publishing Corporation |
| Publication Year: |
2011 |
| Collection: |
University of Zurich (UZH): ZORA (Zurich Open Repository and Archive |
| Subject Terms: |
Institute of Pharmacology and Toxicology; Clinic for Endocrinology and Diabetology; 610 Medicine & health |
| Description: |
Utrophin and dystrophin present two large proteins that link the intracellular actin cytoskeleton to the extracellular matrix via the C-terminal-associated protein complex. Here we describe a novel short N-terminal isoform of utrophin and its protein product in various rat tissues (N-utro, 62 kDa, amino acids 1-539, comprising the actin-binding domain plus the first two spectrin repeats). Using different N-terminal recombinant utrophin fragments, we show that actin binding exhibits pronounced negative cooperativity (affinity constants K(1) = ∼5 × 10(6) and K(2) = ∼1 × 10(5 )M(-1)) and is Ca(2+)-insensitive. Expression of the different fragments in COS7 cells and in myotubes indicates that the actin-binding domain alone binds exlusively to actin filaments. The recombinant N-utro analogue binds in vitro to actin and in the cells associates to the membranes. The results indicate that N-utro may be responsible for the anchoring of the cortical actin cytoskeleton to the membranes in muscle and other tissues. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
English |
| ISSN: |
1110-7243 |
| Relation: |
https://www.zora.uzh.ch/id/eprint/56252/1/Tissue_expression_and_actin_binding-RZ.pdf; info:pmid/22228988; urn:issn:1110-7243 |
| DOI: |
10.1155/2011/904547 |
| Availability: |
https://www.zora.uzh.ch/id/eprint/56252/; https://doi.org/10.1155/2011/904547 |
| Rights: |
info:eu-repo/semantics/openAccess ; Creative Commons: Attribution 3.0 Unported (CC BY 3.0) ; http://creativecommons.org/licenses/by/3.0/ |
| Accession Number: |
edsbas.99F5BF13 |
| Database: |
BASE |