| Title: |
Subunit-specific isotope labelling of heteromeric complexes using cell-free protein expression: application to the 760 kDa ClpXP molecular machine |
| Authors: |
Audibert, Astrid; Vermot, Annelise; Trauchessec, Mathieu; Giandoreggio, Karine; Fenel, Daphna; Le Roy, Aline; Tymen, Chloee; Moscatello, Basile; Gandy, Lauren; Mas, Caroline; Imbert, Lionel; Boisbouvier, Jerome |
| Contributors: |
European Research Council; Agence Nationale de la Recherche |
| Source: |
RSC Chemical Biology ; volume 7, issue 2, page 260-268 ; ISSN 2633-0679 |
| Publisher Information: |
Royal Society of Chemistry (RSC) |
| Publication Year: |
2026 |
| Description: |
An optimised, economical cell-free protocol for producing perdeuterated, methyl-labelled samples of the ClpXP machine, with separate labelling of the ClpX and ClpP components, enables the acquisition of high-resolution 2D methyl-TROSY NMR spectra. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1039/d5cb00259a |
| Availability: |
https://doi.org/10.1039/d5cb00259a; http://pubs.rsc.org/en/content/articlepdf/2026/CB/D5CB00259A |
| Rights: |
http://creativecommons.org/licenses/by-nc/3.0/ |
| Accession Number: |
edsbas.9A67F418 |
| Database: |
BASE |