| Title: |
Novel α-amylase inhibitor hemi-pyocyanin produced by microbial conversion of chitinous discards |
| Authors: |
Nguyen TH; Wang SL; Nguyen AD; Doan MD; Tran TN; Doan CT; Nguyen VB |
| Publication Year: |
2022 |
| Collection: |
Tamkang University Institutional Repository (TKUIR) / 淡江大學機構典藏 |
| Subject Terms: |
α-amylase inhibitors; diabetes; hemi-pyocyanin; marine discards; microbial conversion |
| Description: |
α-Amylase inhibitors (aAIs) have been applied for the efficient management of type 2 diabetes. The aim of this study was to search for potential aAIs produced by microbial fermentation. Among various bacterial strains, Pseudomonas aeruginosa TUN03 was found to be a potential aAI-producing strain, and shrimp heads powder (SHP) was screened as the most suitable C/N source for fermentation. P. aeruginosa TUN03 exhibited the highest aAIs productivity (3100 U/mL) in the medium containing 1.5% SHP with an initial pH of 7–7.5, and fermentation was performed at 27.5 °C for two days. Further, aAI compounds were investigated for scaled-up production in a 14 L-bioreactor system. The results revealed a high yield (4200 U/mL) in a much shorter fermentation time (12 h) compared to fermentation in flasks. Bioactivity-guided purification resulted in the isolation of one major target compound, identified as hemi-pyocyanin (HPC) via gas chromatography-mass spectrometry and nuclear magnetic resonance. Its purity was analyzed by high-performance liquid chromatography. HPC demonstrated potent α-amylase inhibitory activity comparable to that of acarbose, a commercial antidiabetic drug. Notably, HPC was determined as a new aAI. The docking study indicated that HPC inhibits α-amylase by binding to amino acid Arg421 at the biding site on enzyme α-amylase with good binding energy (−9.3 kcal/mol) and creating two linkages of H-acceptors. ; 補正完畢 ; USA |
| Document Type: |
other/unknown material |
| File Description: |
100 bytes; text/html |
| Language: |
English |
| Relation: |
Marine Drugs 20(5), p.283; 全文連結 https://www.mdpi.com/1660-3397/20/5/283; https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/124221; https://tkuir.lib.tku.edu.tw/dspace/bitstream/987654321/124221/1/index.html |
| DOI: |
10.3390/md20050283 |
| Availability: |
https://tkuir.lib.tku.edu.tw/dspace/handle/987654321/124221; https://doi.org/10.3390/md20050283; https://tkuir.lib.tku.edu.tw/dspace/bitstream/987654321/124221/1/index.html |
| Accession Number: |
edsbas.9BD89A2C |
| Database: |
BASE |