| Title: |
Toward the isolation of the D gene controlling the acidity of the peach fruit by positional cloning |
| Authors: |
Boudehri, Karima; Belka, Mohamed Amine; Cardinet, Gaëlle; Capdeville, Gaëlle; Renaud, Christel; Tauzin, Yves; Dirlewanger, Elisabeth; Troadec, Christelle; Jublot, Delphine; Caboche, Michel; Bendahmane, Abdelhafid, A.; Moing, Annick |
| Contributors: |
Unité de recherches Espèces Fruitières et Vigne (UREFV); Institut National de la Recherche Agronomique (INRA); Unité de recherche en génomique végétale (URGV); Institut National de la Recherche Agronomique (INRA)-Université d'Évry-Val-d'Essonne (UEVE)-Centre National de la Recherche Scientifique (CNRS); Station de physiologie végétale |
| Source: |
12. Eucarpia Symposium on Fruit Breeding and Genetics; https://hal.inrae.fr/hal-05286031; 12. Eucarpia Symposium on Fruit Breeding and Genetics, Sep 2007, Zaragoza, Spain. ⟨10.17660/ActaHortic.2009.814.85⟩ |
| Publisher Information: |
CCSD |
| Publication Year: |
2007 |
| Collection: |
Université d'Évry-Val-d'Essonne: HAL |
| Subject Terms: |
CRUCIFERAE; ARABIDOPSIS THALIANA; ROSACEAE; PRUNUS; AFLP; PECHER; CARTOGRAPHIE GENETIQUE; [SDV]Life Sciences [q-bio] |
| Subject Geographic: |
Zaragoza; Spain |
| Description: |
International audience ; The sugar/acid ratio is an essential component of the organoleptic quality for fruits in the Rosaceae family. The D gene, controlling the non-acid trait in peach, is dominant and segregates as a Mendelian character. Using a mapping progeny of 208 F2 individuals derived from a cross between two peach varieties ‘Ferjalou Jalousia®‘ and ‘Fantasia’, the D gene was mapped on linkage group 5. Using the BSA-AFLP method with 960 primer combinations, 12 AFLP markers were identified within 10 cM around the D gene. Two of them co-segregate with the D gene. The fine mapping of the region around the D gene, based on the analyses of an extended F2 progeny of 1510 seedlings, is in progress. Three AFLPs were transformed into codominant SCARs and will be used to screen a new BAC library obtained from the F1 hybrid that is the parent of the F2 progeny. Screening of the BAC library with the flanking markers of the D gene will allow the identification of the clones containing the D and d alleles. |
| Document Type: |
conference object |
| Language: |
English |
| Relation: |
PRODINRA: 12092 |
| DOI: |
10.17660/ActaHortic.2009.814.85 |
| Availability: |
https://hal.inrae.fr/hal-05286031; https://doi.org/10.17660/ActaHortic.2009.814.85 |
| Accession Number: |
edsbas.9BFA5702 |
| Database: |
BASE |