| Title: |
[Untitled] |
| Authors: |
Rawle M. Mcintosh; Claudius Kulvinskas; Nd Donald; B. Kaufman |
| Contributors: |
The Pennsylvania State University CiteSeerX Archives |
| Source: |
http://jmm.sgmjournals.org/content/4/4/535.full.pdf. |
| Collection: |
CiteSeerX |
| Description: |
that nephritogenic streptococci may alter the chemical composition and immunogenicity of immunoglobulin G (IgG) in some patients by enzymatic action or by combination of strepto-coccal products with IgG. The following study was designed to test this hypothesis in vitro. MATERIALS AND METHODS Streptococcus pyogenes type 12, isolated from the pharynx of a patient with acute post-streptococcal glomerulonephritis, was subcultured in Todd-Hewitt broth and incubated at 37°C for 24 hr. The organisms were removed by centrifugation and washed with Dulbecco’s solution, and serial dilutions were plated on blood-agar plates; 1 ml of suspension containing 3 x 104 organisms was used for treatment of human IgG under different conditions. Human IgG was precipitated from pooled human serum with 50 per cent. ammonium sulphate and purified by gel-filtration on Sephadex G 200 (Flodin and Killander, 1962) followed by Chromatography on diethylaminoethyl (DEAE) cellulose (Tomasi and Kunkel, 1964). The IgG thus isolated was centrifuged at 15,000 r.p.m. and the supernatant fluid was tested for purity by immuno-electrophoresis and immunodiffusion in agar gel. The IgG was dialysed extensively against distilled water and was lyophilised. Eleven tests were set up. |
| Document Type: |
text |
| File Description: |
application/pdf |
| Language: |
English |
| Relation: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.603.3740; http://jmm.sgmjournals.org/content/4/4/535.full.pdf |
| Availability: |
http://citeseerx.ist.psu.edu/viewdoc/summary?doi=10.1.1.603.3740; http://jmm.sgmjournals.org/content/4/4/535.full.pdf |
| Rights: |
Metadata may be used without restrictions as long as the oai identifier remains attached to it. |
| Accession Number: |
edsbas.9C29EFA0 |
| Database: |
BASE |