| Title: |
Therapeutic Potential of a Novel Lytic Phage, vB_EclM_ECLFM1, against Carbapenem-Resistant Enterobacter cloacae |
| Authors: |
Saieeda Fabia Ali; Soon-Hian Teh; Hsueh-Hui Yang; Yun-Chan Tsai; Huei-Jen Chao; Si-Shiuan Peng; Shu-Chen Chen; Ling-Chun Lin; Nien-Tsung Lin |
| Source: |
International Journal of Molecular Sciences, Vol 25, Iss 2, p 854 (2024) |
| Publisher Information: |
MDPI AG |
| Publication Year: |
2024 |
| Collection: |
Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: |
Enterobacter cloacae; multidrug resistance; bacteriophage; phage therapy; Karamvirus; antimicrobial resistance; Biology (General); QH301-705.5; Chemistry; QD1-999 |
| Description: |
The global rise of multidrug-resistant Enterobacter cloacae strains, especially those that are resistant to carbapenems and produce metallo-β-lactamases, poses a critical challenge in clinical settings owing to limited treatment options. While bacteriophages show promise in treating these infections, their use is hindered by scarce resources and insufficient genomic data. In this study, we isolated ECLFM1, a novel E. cloacae phage, from sewage water using a carbapenem-resistant clinical strain as the host. ECLFM1 exhibited rapid adsorption and a 15-min latent period, with a burst size of approximately 75 PFU/infected cell. Its genome, spanning 172,036 bp, was characterized and identified as a member of Karamvirus . In therapeutic applications, owing to a high multiplicity of infection, ECLFM1 showed increased survival in zebrafish infected with E. cloacae . This study highlights ECLFM1’s potential as a candidate for controlling clinical E. cloacae infections, which would help address challenges in treating multidrug-resistant strains and contribute to the development of alternative treatments. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://www.mdpi.com/1422-0067/25/2/854; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067; https://doaj.org/article/b940674e28e94264958a4c77f37fbed7 |
| DOI: |
10.3390/ijms25020854 |
| Availability: |
https://doi.org/10.3390/ijms25020854; https://doaj.org/article/b940674e28e94264958a4c77f37fbed7 |
| Accession Number: |
edsbas.9CBC67BD |
| Database: |
BASE |