| Title: |
Tissue-Oxygen-Adaptation of Bone Marrow-Derived Mesenchymal Stromal Cells Enhances Their Immunomodulatory and Pro-Angiogenic Capacity, Resulting in Accelerated Healing of Chemical Burns |
| Authors: |
Marina V. Volkova; Ningfei Shen; Anna Polyanskaya; Xiaoli Qi; Valery V. Boyarintsev; Elena V. Kovaleva; Alexander V. Trofimenko; Gleb I. Filkov; Alexandre V. Mezentsev; Sergey P. Rybalkin; Mikhail O. Durymanov |
| Source: |
International Journal of Molecular Sciences, Vol 24, Iss 4, p 4102 (2023) |
| Publisher Information: |
MDPI AG |
| Publication Year: |
2023 |
| Collection: |
Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: |
injury; burn model; mesenchymal stem cell; hypoxia; cellular respiration; Biology (General); QH301-705.5; Chemistry; QD1-999 |
| Description: |
Transplantation of mesenchymal stromal cells (MSCs) provides a powerful tool for the management of multiple tissue injuries. However, poor survival of exogenous cells at the site of injury is a major complication that impairs MSC therapeutic efficacy. It has been found that tissue-oxygen adaptation or hypoxic pre-conditioning of MSCs could improve the healing process. Here, we investigated the effect of low oxygen tension on the regenerative potential of bone-marrow MSCs. It turned out that incubation of MSCs under a 5% oxygen atmosphere resulted in increased proliferative activity and enhanced expression of multiple cytokines and growth factors. Conditioned growth medium from low-oxygen-adapted MSCs modulated the pro-inflammatory activity of LPS-activated macrophages and stimulated tube formation by endotheliocytes to a much higher extent than conditioned medium from MSCs cultured in a 21% oxygen atmosphere. Moreover, we examined the regenerative potential of tissue-oxygen-adapted and normoxic MSCs in an alkali-burn injury model on mice. It has been revealed that tissue-oxygen adaptation of MSCs accelerated wound re-epithelialization and improved the tissue histology of the healed wounds in comparison with normoxic MSC-treated and non-treated wounds. Overall, this study suggests that MSC adaptation to ‘physiological hypoxia’ could be a promising approach for facilitating skin injuries, including chemical burns. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://www.mdpi.com/1422-0067/24/4/4102; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067; https://doaj.org/article/6f722ec282a64512bae215ed3cb3de3c |
| DOI: |
10.3390/ijms24044102 |
| Availability: |
https://doi.org/10.3390/ijms24044102; https://doaj.org/article/6f722ec282a64512bae215ed3cb3de3c |
| Accession Number: |
edsbas.9D8EDC6C |
| Database: |
BASE |