| Title: |
Minor Isozymes Tailor Yeast Metabolism to Carbon Availability |
| Authors: |
Bradley, Patrick H.; Gibney, Patrick A.; Botstein, David; Troyanskaya, Olga G.; Rabinowitz, Joshua D. |
| Contributors: |
McCue, Lee Ann; Department of Energy; HHS | National Institutes of Health; National Science Foundation; DOD | USAF | AFMC | Air Force Office of Scientific Research |
| Source: |
mSystems ; volume 4, issue 1 ; ISSN 2379-5077 |
| Publisher Information: |
American Society for Microbiology |
| Publication Year: |
2019 |
| Description: |
Gene duplication is one of the main evolutionary paths to new protein function. Typically, duplicated genes either accumulate mutations and degrade into pseudogenes or are retained and diverge in function. Some duplicated genes, however, show long-term persistence without apparently acquiring new function. An important class of isozymes consists of those that catalyze the same reaction in the same compartment, where knockout of one isozyme causes no known functional defect. Here we present an approach to assigning specific functional roles to seemingly redundant isozymes. First, gene expression data are analyzed computationally to identify conditions under which isozyme expression diverges. Then, knockouts are compared under those conditions. This approach revealed that the expression of many yeast isozymes diverges in response to carbon availability and that carbon source manipulations can induce fitness phenotypes for seemingly redundant isozymes. A driver of these fitness phenotypes is differential allosteric enzyme regulation, indicating isozyme divergence to achieve more-optimal control of metabolism. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1128/msystems.00170-18 |
| Availability: |
https://doi.org/10.1128/msystems.00170-18; https://journals.asm.org/doi/pdf/10.1128/msystems.00170-18 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ ; https://journals.asm.org/non-commercial-tdm-license |
| Accession Number: |
edsbas.9F0AF6C9 |
| Database: |
BASE |