| Title: |
Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells |
| Authors: |
Young, FI; Telezhkin, V; Youde, SJ; Langley, MS; Stack, M; Kemp, PJ; Waddington, RJ; Sloan, AJ; Song, B |
| Contributors: |
Huang, G |
| Publisher Information: |
HINDAWI LTD |
| Publication Year: |
2016 |
| Collection: |
The University of Melbourne: Digital Repository |
| Description: |
Cellular heterogeneity presents an important challenge to the development of cell-based therapies where there is a fundamental requirement for predictable and reproducible outcomes. Transplanted Dental Pulp Stem/Progenitor Cells (DPSCs) have demonstrated early promise in experimental models of spinal cord injury and stroke, despite limited evidence of neuronal and glial-like differentiation after transplantation. Here, we report, for the first time, on the ability of single cell-derived clonal cultures of murine DPSCs to differentiate in vitro into immature neuronal-like and oligodendrocyte-like cells. Importantly, only DPSC clones with high nestin mRNA expression levels were found to successfully differentiate into Map2 and NF-positive neuronal-like cells. Neuronally differentiated DPSCs possessed a membrane capacitance comparable with primary cultured striatal neurons and small inward voltage-activated K(+) but not outward Na(+) currents were recorded suggesting a functionally immature phenotype. Similarly, only high nestin-expressing clones demonstrated the ability to adopt Olig1, Olig2, and MBP-positive immature oligodendrocyte-like phenotype. Together, these results demonstrate that appropriate markers may be used to provide an early indication of the suitability of a cell population for purposes where differentiation into a specific lineage may be beneficial and highlight that further understanding of heterogeneity within mixed cellular populations is required. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| ISSN: |
1687-966X |
| Relation: |
Young, F. I., Telezhkin, V., Youde, S. J., Langley, M. S., Stack, M., Kemp, P. J., Waddington, R. J., Sloan, A. J. & Song, B. (2016). Clonal Heterogeneity in the Neuronal and Glial Differentiation of Dental Pulp Stem/Progenitor Cells. Stem Cells International, 2016 (1), https://doi.org/10.1155/2016/1290561.; https://hdl.handle.net/11343/245524 |
| Availability: |
https://hdl.handle.net/11343/245524 |
| Rights: |
https://creativecommons.org/licenses/by/4.0 ; CC BY |
| Accession Number: |
edsbas.9FAE35CB |
| Database: |
BASE |