| Title: |
Identification of Neotropical Culex Mosquitoes by MALDI-TOF MS Profiling |
| Authors: |
Costa, Monique Melo; Guidez, Amandine; Briolant, Sébastien; Talaga, Stanislas; Issaly, Jean; Naroua, Halima; Carinci, Romuald; Gaborit, Pascal; Lavergne, Anne; Dusfour, Isabelle; Duchemin, Jean-Bernard; Almeras, Lionel |
| Contributors: |
Institut de Recherche Biomédicale des Armées Antenne Marseille (IRBA); Vecteurs - Infections tropicales et méditerranéennes (VITROME); Institut de Recherche pour le Développement (IRD)-Aix Marseille Université (AMU)-Institut de Recherche Biomédicale des Armées Brétigny-sur-Orge (IRBA); Institut Hospitalier Universitaire Méditerranée Infection (IHU Marseille); Entomologie médicale Cayenne, Guyane française; Institut Pasteur de la Guyane; Pasteur Network (Réseau International des Instituts Pasteur)-Pasteur Network (Réseau International des Instituts Pasteur); Centre de Recherche Médicale et Sanitaire Niamey, Niger (CERMES); Pasteur Network (Réseau International des Instituts Pasteur); Laboratoire des Interactions Virus-Hôtes Cayenne, Guyane Française; This work has been supported by the Délégation Générale pour l’Armement (DGA, MSProfileR project, Grant no PDH-2-NBC 2-B-2201) and by the Regional Health Agency of French Guiana (Conventions n° FIR/ARS/2014/91, n°FIR/ARS/2015/68, and Convention ARS/IPG 2021–2023—26th November 2021). This work was also partially supported by the European Union FEDER controle Programme under Grant number GY0010695. |
| Source: |
ISSN: 2414-6366 ; Tropical Medicine and Infectious Disease ; https://amu.hal.science/hal-04115281 ; Tropical Medicine and Infectious Disease, 2023, 8 (3), pp.168. ⟨10.3390/tropicalmed8030168⟩. |
| Publisher Information: |
CCSD; MDPI |
| Publication Year: |
2023 |
| Collection: |
Aix-Marseille Université: HAL |
| Subject Terms: |
Culex species; neotropical mosquito; French Guiana; MALDI-TOF MS; proteomic tool; arbovirus; monitoring; [SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology; [SDV.MHEP.CSC]Life Sciences [q-bio]/Human health and pathology/Cardiology and cardiovascular system; [SDV.MHEP.ME]Life Sciences [q-bio]/Human health and pathology/Emerging diseases; [SDV.MHEP.MI]Life Sciences [q-bio]/Human health and pathology/Infectious diseases; [SDV.MP.PAR]Life Sciences [q-bio]/Microbiology and Parasitology/Parasitology; [SDV.MP.VIR]Life Sciences [q-bio]/Microbiology and Parasitology/Virology |
| Description: |
International audience ; The mosquito (Diptera: Culicidae) fauna of French Guiana encompasses 242 species, of which nearly half of them belong to the genus Culex. Whereas several species of Culex are important vectors of arboviruses, only a limited number of studies focus on them due to the difficulties to morphologically identify field-caught females. Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has been reported as a promising method for the identification of mosquitoes. Culex females collected in French Guiana were morphologically identified and dissected. Abdomens were used for molecular identification using the COI (cytochrome oxidase 1) gene. Legs and thorax of 169 specimens belonging to 13 Culex species, (i.e., Cx. declarator, Cx. nigripalpus, Cx. quinquefasciatus, Cx. usquatus, Cx. adamesi, Cx. dunni, Cx. eastor, Cx. idottus, Cx. pedroi, Cx. phlogistus, Cx. portesi, Cx. rabanicolus and Cx. spissipes) were then submitted to MALDI-TOF MS analysis. A high intra-species reproducibility and inter-species specificity of MS spectra for each mosquito body part tested were obtained. A corroboration of the specimen identification was revealed between MALDI-TOF MS, morphological and molecular results. MALDI-TOF MS protein profiling proves to be a suitable tool for identification of neotropical Culex species and will permit the enhancement of knowledge on this highly diverse genus. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/36977169; PUBMED: 36977169; PUBMEDCENTRAL: PMC10055718 |
| DOI: |
10.3390/tropicalmed8030168 |
| Availability: |
https://amu.hal.science/hal-04115281; https://amu.hal.science/hal-04115281v1/document; https://amu.hal.science/hal-04115281v1/file/tropicalmed-08-00168.pdf; https://doi.org/10.3390/tropicalmed8030168 |
| Rights: |
https://creativecommons.org/licenses/by/4.0/ ; info:eu-repo/semantics/OpenAccess |
| Accession Number: |
edsbas.9FE4A710 |
| Database: |
BASE |