| Title: |
[In Press] Exploring the broad-spectrum pharmacological activity of two less studied Australian native fruits : chemical characterisation using LCMS-driven metabolomics |
| Authors: |
Dissanayake, Indeewarie Hemamali (S36441); Alsherbiny, Muhammad (S34387); Stack, Colin M. (R12090); Chang, Dennis Hsu-Tung (R7407); Li , Chun Guang (R17249); Kaur, Kirandeep (S35440); Bhuyan, Deep Jyoti (R19430) |
| Publisher Information: |
U.K., Royal Society of Chemistry |
| Publication Year: |
2024 |
| Collection: |
University of Western Sydney (UWS): Research Direct |
| Subject Terms: |
300602 - Food chemistry and food sensory science; 280103 - Expanding knowledge in the biomedical and clinical sciences |
| Description: |
Australian fruits such as native currant (Acrotriche depressa) and lemon aspen (Acronychia acidula) are under-examined in terms of their therapeutic potential. We determined the in vitro antiproliferative activity of the water and ethanol extracts of native currant and lemon aspen against MCF7 breast adenocarcinoma cells using the Alamar blue assay. The most potent extracts (native currant water, NC-W; native currant ethanol, NC-Et; lemon aspen ethanol, LA-Et) were further evaluated using flow cytometry to detect the potential induction of apoptosis in MCF7 cells whereas 2’7’- dichlorodihydrofluorescein diacetate (H2DCFDA) assay was implemented to understand the impact of the extracts on the intracellular reactive oxygen species (ROS) levels in the MCF7 cells. Furthermore, the antioxidant activity of the extracts was assessed using ABTS [2,2’-azinobis-(3-ethylbenzothiazoline-6-sulfonate)] and CUPRAC (cupric reducing antioxidant capacity) assays. The antimicrobial susceptibility testing of NC-W, NC-Et, and LA-Et was carried out against Gram-positive (Staphylococcus aureus), Gram-negative (Escherichia coli), and yeast (Candida albicans) strains using a resazurin-based assay. Additionally, potential metabolites in NC-W and NC-Et extracts were analysed with liquid chromatography-mass spectrometry (LC-MS) driven metabolomics and chemometrics to spot differential and major metabolites. A dose-dependent antiproliferative activity was conferred by the NC extracts against MCF7 cells. Of the two LA extracts, only LA-Et showed a dose-dependent antiproliferative activity at higher concentrations. Both NC extracts and LA-Et induced apoptosis in MCF7 cells. None of the extracts increased the production of ROS significantly in MCF7 cells compared to the untreated control. A dose-dependent antioxidant activity was observed in both antioxidant assays. Both NC and LA extracts showed a similar minimum inhibitory concentration (MIC) value against S. aureus. Only LA-Et showed activity against E. coli, while NC-W and NC-Et were less ... |
| Document Type: |
article in journal/newspaper |
| File Description: |
print |
| Language: |
English |
| Relation: |
Food and Function--2042-6496--2042-650X Vol. Issue. No. pp: - |
| DOI: |
10.1039/D4FO01155D |
| Availability: |
https://doi.org/10.1039/D4FO01155D; https://hdl.handle.net/1959.7/uws:76883 |
| Accession Number: |
edsbas.A2F053AD |
| Database: |
BASE |