| Title: |
Digital Microfluidic Hemagglutination Assays for Blood Typing, Donor Compatibility Testing, and Hematocrit Analysis |
| Authors: |
Sklavounos, Alexandros A; Lamanna, Julian; Modi, Dimpy; Gupta, Sidharth; Mariakakis, Alex; Callum, Jeannie; Wheeler, Aaron R |
| Contributors: |
Natural Sciences and Engineering Research Council of Canada; Abbott Diagnostics; Canadian Blood Services |
| Source: |
Clinical Chemistry ; volume 67, issue 12, page 1699-1708 ; ISSN 0009-9147 1530-8561 |
| Publisher Information: |
Oxford University Press (OUP) |
| Publication Year: |
2021 |
| Description: |
Background Blood typing, donor compatibility testing, and hematocrit analysis are common tests that are important in many clinical applications, including those found in high-stakes settings such as the trauma center. These tests are typically performed in centralized laboratories with sample batching; the minutes that are lost in this mode can lead to adverse outcomes, especially for critical-care patients. As a step toward providing rapid results at the bedside, we developed a point-of-care hemagglutination system relying on digital microfluidics (DMF) and a unique, automated readout tool, droplet agglutination assessment using digital microfluidics (DAAD). Methods ABO and Rhesus blood grouping, donor crossmatching, and hematocrit assays were developed on a portable DMF platform that allowed for automated sample processing. The result of each assay could be determined by eye or automatically with the DAAD imaging tool. Results DMF-DAAD was applied to 109 samples collected from different sources (including commercial samples, pinpricks from volunteers, and a hospital blood bank), with perfect fidelity to gold-standard results. Some of these tests were carried out by a nonexpert in a hospital trauma center. Proof-of-concept results were also collected from smaller sample sets for donor compatibility testing and hematocrit analysis. Conclusion DMF-DAAD shows promise for delivering rapid, reliable results in a format well suited for a trauma center and other settings where every minute counts. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| DOI: |
10.1093/clinchem/hvab180 |
| DOI: |
10.1093/clinchem/hvab180/40452957/hvab180.pdf |
| Availability: |
https://doi.org/10.1093/clinchem/hvab180; http://academic.oup.com/clinchem/advance-article-pdf/doi/10.1093/clinchem/hvab180/40452957/hvab180.pdf; https://academic.oup.com/clinchem/article-pdf/67/12/1699/41387953/hvab180.pdf |
| Rights: |
https://academic.oup.com/journals/pages/open_access/funder_policies/chorus/standard_publication_model |
| Accession Number: |
edsbas.A321D1A0 |
| Database: |
BASE |