| Title: |
The first reptilian allergen and major allergen for fish-allergic patients: Crocodile β-parvalbumin |
| Authors: |
Ruethers, Thimo; Nugraha, Roni; Taki, Aya C.; O'Malley, Andrea; Karnaneedi, Shaymaviswanathan; Zhang, Stephanie; Kapingidza, A. Brenda; Mehr, Sam; Kamath, Sandip D.; Chruszcz, Maksymilian; Mackay, Graham; Campbell, Dianne E.; Lopata, Andreas L. |
| Publisher Information: |
Wiley-Blackwell |
| Publication Year: |
2022 |
| Collection: |
James Cook University, Australia: ResearchOnline@JCU |
| Description: |
Background: Clinical cross-reactivity between bony fish, cartilaginous fish, frog, and chicken muscle has previously been demonstrated in fish-allergic patients. In indicative studies, two reports of anaphylaxis following the consumption of crocodile meat and IgE-cross-binding were linked to the major fish allergen parvalbumin (PV). This study investigates IgE-binding proteins in crocodile meat with a focus on PV and their clinical relevance. Methods: Proteins were extracted from muscle tissue of crocodile, three bony fish, and two cartilaginous fish. A cohort of fish-allergic pediatric patients (n = 77) underwent allergen skin prick testing (SPT) to three fish preparations (n = 77) and crocodile (n = 12). IgE-binding proteins were identified and quantified by SDS-PAGE, mass spectrometric analyses, and immunoblotting using commercial and in-house antibodies, as well as individual and pooled patients’ serum. PV isoforms were purified or recombinantly expressed before immunological analyses, including human mast cell degranulation assay. Results: Of the tissues analyzed, PV was most abundant in heated crocodile preparation, triggering an SPT of ≥3 mm in 8 of 12 (67%) fish-allergic patients. Seventy percent (31 of 44) of fish PV-sensitized patients demonstrated IgE-binding to crocodile PV. Crocodile β-PV was the major IgE-binding protein but 20-fold less abundant than α-PV. Cellular reactivity was demonstrated for β-PV and epitopes predicted, explaining frequent IgE-cross-binding of β-PVs. Both PV isoforms are now registered as the first reptile allergens with the WHO/IUIS (β-PV as Cro p 1 and α-PV as Cro p 2). Conclusion: Fish-allergic individuals may be at risk of an allergy to crocodile and should seek specialist advice before consuming crocodilian meat. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
unknown |
| Relation: |
https://doi.org/10.1111/pai.13781; https://researchonline.jcu.edu.au/74420/; https://researchonline.jcu.edu.au/74420/1/74420.pdf |
| Availability: |
https://researchonline.jcu.edu.au/74420/1/74420.pdf |
| Rights: |
open |
| Accession Number: |
edsbas.A359464 |
| Database: |
BASE |