| Title: |
Development of a MS compatible HPLC-HILIC method for the analysis of allantoin and glycolic acid in snail slime and related dosage forms: Focus on the enantioseparation of allantoin |
| Authors: |
Bonafè S.; Protti M.; Carotti A.; Pagano C.; Di Michele A.; Migni A.; Perioli L.; Mercolini L.; Sardella R. |
| Contributors: |
Bonafè, S.; Protti, M.; Carotti, A.; Pagano, C.; Di Michele, A.; Migni, A.; Perioli, L.; Mercolini, L.; Sardella, R. |
| Publication Year: |
2025 |
| Collection: |
IRIS Università degli Studi di Bologna (CRIS - Current Research Information System) |
| Subject Terms: |
Amylose-based chiral stationary phase; Diol-type stationary phase; Enantioselective analysi; Hydrophilic interaction liquid chromatography; Snail slime-based hydrogel |
| Description: |
Glycolic acid and allantoin are known to be responsible, at least in part, for the main activities of snail slime, such as moisturizing, skin regeneration, antioxidant, soothing and anti-inflammatory. Accordingly, a hydrophilic interaction liquid chromatography (HILIC) method for the analysis of allantoin and glycolic acid in snail slime samples was developed by a conventional UV-Vis detector and the diol-type Luna (R) HILIC column. An optimized eluent made up of acetonitrile/water/methanol/formic acid (90:5:5:0.1 v/v/v/v) allowed the separation of the two investigated compounds from each other and from lactic acid and hydantoin used as internal standards. Being allantoin a chiral compound, an enantioselective HPLC protocol was also developed. The chiral analysis of allantoin was performed with the Lux (R) 3 mu m i-Amilose-3 chiral stationary phase using the same mobile phase as for the achiral analysis. The chiral method was efficiently transferred to a HPLC system coupled to triplequadrupole mass spectrometry (MS/MS). HPLC-MS/MS analysis revealed the racemic nature of allantoin in the pure snail slime. The same profile was also observed in a prototype hydrogel prepared with snail slime. Moreover, the same method allowed to measure the amount of glycolic acid in the two investigated samples (pure snail slime and gel) and ascertain a valuable extraction recovery. The enantiomeric elution order (S) |
| Document Type: |
article in journal/newspaper |
| File Description: |
ELETTRONICO |
| Language: |
English |
| Relation: |
info:eu-repo/semantics/altIdentifier/pmid/40023099; info:eu-repo/semantics/altIdentifier/wos/WOS:001438003700001; volume:290; firstpage:1; lastpage:7; numberofpages:7; journal:TALANTA; https://hdl.handle.net/11585/1009760; https://doi.org/10.1016/j.talanta.2025.127832 |
| DOI: |
10.1016/j.talanta.2025.127832 |
| Availability: |
https://hdl.handle.net/11585/1009760; https://doi.org/10.1016/j.talanta.2025.127832 |
| Rights: |
info:eu-repo/semantics/openAccess ; license:Licenza per Accesso Aperto. Creative Commons Attribuzione (CCBY) ; license uri:iris.PUB15 |
| Accession Number: |
edsbas.A36CC273 |
| Database: |
BASE |