| Title: |
Nonadenylylated mRNA is present as polyadenylylated RNA in nuclei of Drosophila. |
| Authors: |
Zimmerman, JL; Fouts, DL; Levy, LS; Manning, JE |
| Source: |
Proceedings of the National Academy of Sciences of the United States of America, vol 79, iss 10 |
| Publisher Information: |
eScholarship, University of California |
| Publication Year: |
1982 |
| Collection: |
University of California: eScholarship |
| Subject Terms: |
31 Biological Sciences (for-2020); 3105 Genetics (for-2020); Genetics (rcdc); Animals (mesh); Base Sequence (mesh); Cell Nucleus (mesh); Drosophila melanogaster (mesh); Larva (mesh); Molecular Weight (mesh); Nucleic Acid Hybridization (mesh); Poly A (mesh); RNA; Messenger (mesh) |
| Time: |
3148 - 3152 |
| Description: |
The sequence complexity of nuclear total RNA and nuclear poly(A)+RNA from Drosophila third-instar larvae was determined by hybridization of these RNAs to labeled single-copy DNA. At saturation, the nuclear poly(A)+ - and total RNA hybridized to 11% and 22.5% of the single-copy DNA, respectively. The increase in complexity of nuclear total RNA over that observed for nuclear poly(A)+RNA indicates the presence of a discrete class of nonoadenylylated nuclear RNA molecules. The relationship between DNA sequences coding for nuclear RNA and mRNA was then determined by hybridization of nuclear total and poly(A)+RNA to DNA enriched for mRNA coding sequences. The results of these studies show that those single-copy DNA sequences that are represented in either the poly(A)+ - or poly(A)- mRNA population are transcribed into RNA molecules that appear in the nuclear poly(A)+RNA population. |
| Document Type: |
article in journal/newspaper |
| File Description: |
application/pdf |
| Language: |
unknown |
| Relation: |
qt35r5q2np; https://escholarship.org/uc/item/35r5q2np; https://escholarship.org/content/qt35r5q2np/qt35r5q2np.pdf |
| DOI: |
10.1073/pnas.79.10.3148 |
| Availability: |
https://escholarship.org/uc/item/35r5q2np; https://escholarship.org/content/qt35r5q2np/qt35r5q2np.pdf; https://doi.org/10.1073/pnas.79.10.3148 |
| Rights: |
CC-BY |
| Accession Number: |
edsbas.A3D3E2A6 |
| Database: |
BASE |