| Title: |
Olive Oil Extracts and Oleic Acid Attenuate the LPS-Induced Inflammatory Response in Murine RAW264.7 Macrophages but Induce the Release of Prostaglandin E2 |
| Authors: |
Anke Katharina Müller; Franziska Albrecht; Carsten Rohrer; Andreas Koeberle; Oliver Werz; Wiebke Schlörmann; Michael Glei; Stefan Lorkowski; Maria Wallert |
| Source: |
Nutrients, Vol 13, Iss 12, p 4437 (2021) |
| Publisher Information: |
MDPI AG |
| Publication Year: |
2021 |
| Collection: |
Directory of Open Access Journals: DOAJ Articles |
| Subject Terms: |
olive oil; oleic acid; inflammation; macrophages; phospholipid incorporation; Nutrition. Foods and food supply; TX341-641 |
| Description: |
Olive oil contains high amounts of oleic acid (OA). Although OA has been described to inhibit inflammatory processes, the effects of olive oil on cellular mechanisms remain poorly understood. Therefore, we compared the effects of major fatty acids (FA) from olive oil with those of olive oil extracts (OOE) on inflammatory mediators and alterations in the cellular phospholipid composition in murine macrophages. Upon treatment with different OOE, FA compositions of lipopolysaccharide (LPS)-stimulated murine RAW264.7 macrophages were analyzed using gas chromatography. Olive oil extracts and OA significantly reduced the LPS-induced expression of inducible nitric oxide synthase (iNos), cyclooxygenase (Cox2), and interleukin-6 mRNA. In addition, a significant decrease in Cox2 and iNos protein expression was observed. The formation of nitric oxide was significantly reduced, while the formation of prostaglandin (PG) E 2 from arachidonic acid significantly increased after treatment with OOE or OA. The latter was associated with a shift in the phospholipid FA composition from arachidonic acid to OA, resulting in an elevated availability of arachidonic acid. Together, OOE and OA mediate anti-inflammatory effects in vitro but increase the release of arachidonic acid and hereinafter PGE 2 , likely due to elongation of OA and competitive incorporation of fatty acids into membrane phospholipids. |
| Document Type: |
article in journal/newspaper |
| Language: |
English |
| Relation: |
https://www.mdpi.com/2072-6643/13/12/4437; https://doaj.org/toc/2072-6643; https://doaj.org/article/acab989e28f645508ec1c6b8cd208b93 |
| DOI: |
10.3390/nu13124437 |
| Availability: |
https://doi.org/10.3390/nu13124437; https://doaj.org/article/acab989e28f645508ec1c6b8cd208b93 |
| Accession Number: |
edsbas.A4CB295 |
| Database: |
BASE |